食品科学2017，Vol.38Issue(4)：283-289,7.DOI:10.7506/spkx1002-6630-201704046
基于16S rRNA基因DNA条形码鉴定美洲鳗、欧洲鳗、日本鳗
Identification of Anguilla rostrata, Anguilla anguilla and Anguilla japonica Using DNA Barcoding Based on 16S rRNA Gene
摘要
Abstract
To meet the demands of enterprises engaged in eel farming,processing and trading and law enforcements,a DNA barcoding method for the identification of three main farmed eel species,Anguilla rostrata,A.Anguilla and A.japonica,was developed.Universal 16S rRNA primers were designed and used to amplify partial 16S rRNA gene fragments ofA.rostrata,A4.Anguilla and A.japonica and their PCR products were sequenced to be 638,638 and 640 bp in length,respectively.The specific DNA fragment (243 bp) was selected as standard DNA barcode with eel species specificity to identify eel species conveniently,and a forward primer (22 bp) and a reversed primer (23 bp) were designed and used to establish the PCR-DNA barcoding method.The results of the application of the proposed method over the past three years showed that it is convenient,accurate,stable,and useful to identify the three eel species.关键词
美洲鳗/欧洲鳗/日本鳗/16S rRNA基因/DNA条形码/鉴定Key words
A.rostrata/A.Anguilla/A.japonica/16S rRNA gene/DNA barcode/identification分类
农业科技引用本文复制引用
陈文炳,缪婷玉,彭娟,邵碧英,陈彬,江树勋,张志灯..基于16S rRNA基因DNA条形码鉴定美洲鳗、欧洲鳗、日本鳗[J].食品科学,2017,38(4):283-289,7.基金项目
福建省科技计划农业引导性项目(2015N0016) （2015N0016）
