中国食品添加剂Issue(2)：106-111,6.
产壳聚糖酶菌株的筛选、鉴定及发酵条件优化
Screeningand identification of a chitosanase-producing strain and optimization of its fermentation conditions
刘阳 1赵华 1郑雅元 1田梁 1李燕1
作者信息
- 1. 天津科技大学生物工程学院,工业微生物教育部重点实验室,天津300457
- 折叠
摘要
Abstract
Eight strains with high chitosanase activity were screened from the sludge of shrimp pond by transparent circle method using at Chitosan as the sole carbon source.After further screening,a testing strain of TCCC 150018 was selected and its chitosanase activity was 2.48U / mL.By morphological observation and 16SrDNA sequence analysis,the strain TCCC150018 was identified as Bacillus cereus.Based on the results from single factor test,response surface methodology (RSM) combined with central composite design was used to optimize cultivation conditions of Bacillus cereus TCCC150018.The optimal conditions were achieved to be yeast extract powder 16g/L,glucose 11.5g/L,Twain-80 1.2g/L.Under the optimal conditions,the activity of chitosanase was 4.92U/mL.It was 1.98 times increased on chitosanase activity than the control one.The study has a good prospect in industrial production.关键词
筛菌/壳聚糖酶/蜡状芽孢杆菌/非诱导型酶/响应面法优化Key words
strain screening/chitosanase/bacillus cereus/non-inducible enzyme/response surface analysis分类
生物科学引用本文复制引用
刘阳,赵华,郑雅元,田梁,李燕..产壳聚糖酶菌株的筛选、鉴定及发酵条件优化[J].中国食品添加剂,2017,(2):106-111,6.
