中国食品学报2017，Vol.17Issue(1)：77-83,7.DOI:10.16429/j.1009-7848.2017.01.010
脂肪酶催化玉米胚芽油与辛酸制备MLM型结构脂质
Lipase-catalyzed Acidolysis of Maize Germ Oil with Caprylic Acid to Produce MLM-type Structured Lipids
摘要
Abstract
In order to produce functional MLM-type structured Lipid with maize germ oil in solvent free system was investigated.Six commercial lipases from different sources (Lipozyme RM IM、Lipozyme TL IM、Novozym 435、Lipase AK、Lipase AY and Newlase F) were compared for their ability to incorporate caprylic acid into the maize germ oil in solvent free system.Of the six lipases that were tested in the initial screening,Lipozyme RM IM from Rhizomucor miehei resulted in the highest incorporation of caprylic acid into maize germ oil.This enzyme was further studied for the effect of mole ratio of oil and caprylic acid,enzyme load,reaction time and reaction temperature on the incorporation of caprylic acid into maize germ oil.Incorporation of caprylic acid was higher when reactions were carried with 12％ lipase of the total weight of substrates with a 1∶4 mole ratio of oil and caprylic acid,time course and temperature for synthesis MLM-type structured lipids were 16 h and 50 ℃.Lipase-catalyzed acidolysis of grape seed oil with caprylic acid could producehigh quality MLM-type structured lipids in solvent free system.关键词
玉米胚芽油/辛酸/脂肪酶/酸解反应/MLM型结构脂质Key words
maize germ oil/caprylic acid/lipase/acidolysis reaction/MLM-type structured lipids引用本文复制引用
王强,贺稚非,李贵节,王存,彭荣,袁君,李洪军..脂肪酶催化玉米胚芽油与辛酸制备MLM型结构脂质[J].中国食品学报,2017,17(1):77-83,7.基金项目
国家自然科学基金面上项目(31671787) （31671787）
国家自然科学基金青年项目(31401559) （31401559）
重庆高校创新团队建设计划资助项目(CXTDX201601040) （CXTDX201601040）
