中国食品学报2017，Vol.17Issue(5)：55-63,9.DOI:10.16429/j.1009-7848.2017.05.008
粗壮脉纹孢菌固态发酵产类胡萝卜素的条件优化及其抗氧化活性
Antioxidant Activity and Optimization of Solid-state Fermentation Condition for Carotenoids by Neurospora crassa
摘要
Abstract
In this paper,the conditions of solid-state fermentation for producing carotenoids were optimized with agricultural by-products by Neurospora crassa,through Plackett-Burman experimental design and response surface method,and the antioxidant capacity of carotenoids were studied.The carotenoids production was 966.21 μg/g when the fermentation were carried out on the optimized medium,which composed of 0.51％ ammonium sulphate,20％ brewer's grain,34.25％ potato residues,45.75％ rice bran (calculated in dry weight) and the initial moisture content is 72.15％,pH 6.5 for 120 h.The results of antioxidant experiments indicated that:compared with common antioxidant,lycopene and β-carotene,the scavenging capability of carotenoids produced by Neurospora crassa towards DPPH,superoxide and hydroxyl radical were stronger in the experimental concentration.In addition,carotenoids produced by Neurospora crassa exhibited high total antioxidant capability and good chelating ability of metals.关键词
粗壮脉纹孢菌/类胡萝卜素/优化/抗氧化Key words
Neurospora crassa/carotenoid/optimization/antioxidant引用本文复制引用
李静,杨建远,杨风玲,何悦铭,邓泽元..粗壮脉纹孢菌固态发酵产类胡萝卜素的条件优化及其抗氧化活性[J].中国食品学报,2017,17(5):55-63,9.基金项目
食品科学与技术国家重点实验室目标导向课题(SKLF-ZZA-201610) （SKLF-ZZA-201610）
江西省教育厅项目(GJJ150186) （GJJ150186）
江西省自然科学基金项目(20161BAB204170) （20161BAB204170）
