现代食品科技2017，Vol.33Issue(6)：19-25,7.DOI:10.13982/j.mfst.1673-9078.2017.6.004
诸葛菜碱Ⅰ对H2O2所致HepG2细胞氧化损伤的保护作用
Protective Effect of Orychophragmuspine Ⅰ Against Oxidative Damage in HepG2 Cells Induced by Hydrogen Peroxide
摘要
Abstract
Orychophragmus violaceus seed extract was isolated and purified by silica gel chromatography,and the structures were identified by spectral analysis and chemical methods.A new compound was identified for the first time and named as Orychophragmuspine Ⅰ.The protective effect and mechanism of Orychophragmuspine Ⅰ on hydrogen peroxide (H2O2)-induced oxidative damage in HepG2 cells was investigated by establishing a model of H2O2-induced cell damage.Before hydrogen peroxide stimulation,HepG2 cells were pretreated with different concentrations of Orychophragmuspine Ⅰ for 12 h,and then cells were treated with 400 μmol/L H2O2 for two hours.The survival rate of cells was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.Lactate dehydrogenase (LDH) activity in the medium,cellular malondialdehyde (MDA) content,and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX)were measured using a microplate method,and nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase-1 (HO-1) protein expression was measured by western blot.The results showed that,compared with the model group,12 h of Orychophragmuspine Ⅰ treatment (53.5,107,and 214 μmol/L) increased the cell survival rate (p＜0.01),significantly reduced the release o f LDH to the extracellular fluid (p＜0.01),decreased MDA content in cells (p＜0.05,p＜0.01),and increased the intracellular activities of SOD and GSH-PX (p＜0.05,p＜0.01) and the level of Nrf2 protein.Therefore,Orychophragmuspine Ⅰ has a protective effect against H2O2-induced oxidative damage in HepG2 cells.关键词
诸葛菜/诸葛菜碱Ⅰ/抗氧化/HeDG2Key words
Orychophragmus violaceus/Orychophragmuspine Ⅰ/antioxidant/HepG2 cells引用本文复制引用
刘晨琪,朱乃亮,杨淑贤,戴应和,李立勇,许旭东,曹丽..诸葛菜碱Ⅰ对H2O2所致HepG2细胞氧化损伤的保护作用[J].现代食品科技,2017,33(6):19-25,7.基金项目
北京市自然科学基金项目(7164281) （7164281）
