现代食品科技2017，Vol.33Issue(6)：26-31,99,7.DOI:10.13982/j.mfst.1673-9078.2017.6.005
不同极性酸浆提取液对体外诱导的非酒精性脂肪肝细胞的影响
Effects of Physalis alkekengi Extracts with Different Polarities on In Vitro Induced Nonalcoholic Fatty Liver Cells
摘要
Abstract
The aim of this study was to investigate the effects of Physalis alkekengi extracts with different polarities in an in vitro model of induced nonalcoholic fatty liver disease (NAFLD) and identify the possible mechanism.Physalis alkekengi samples were extracted using organic solvents with different polarities.The concentrations of medical fat emulsion for injection and Physalis alkekengi extracts were selected by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.Lipid droplets stained with oil red O were observed under a light microscope.The triglyceride (TG),alanine aminotransferase (ALT),aspartate transaminase (AST),and gamma-glutamyl transpeptidase (GGT) levels were determined by an automatic biochemical analyzer.Compared with normal liver cells,HL7702,the model group showed a large quantity of orange-red lipid droplets in the cytoplasm after oil red O staining,and the intracellular TG,ALT,AST,and GGT levels increased significantly (p＜0.01).Compared with the model group,the groups treated with different Physalis alkekengi extracts for 12 h exhibited a considerably reduced number of orange-red lipid droplets in the cytoplasm and significantly decreased levels of intracellular TG,ALT,AST,and GGT (p＜0.01).An in vitro cell model of nonalcoholic fatty liver disease was successfully established,and treatment with Physalis alkekengi exraacts with different polarities could reduce the accumulation of TG and leakage ofALT,AST,and GGT in NAFLD cells.关键词
不同酸浆提取物/非酒精性脂肪肝/医用脂肪乳Key words
Physalis alkekengi extracts with different polarities/nonalcoholic fatty liver/medical fat emulsion引用本文复制引用
刘敏,于洋,雷钧涛,郗艳丽,张岚,马洪波,王舒然..不同极性酸浆提取液对体外诱导的非酒精性脂肪肝细胞的影响[J].现代食品科技,2017,33(6):26-31,99,7.基金项目
吉林省教育厅“十二五”科学技术研究项目支助项目(吉教科合字[2013]第360号) （吉教科合字[2013]第360号）
吉林省卫生厅支助项目(2014ZC063) （2014ZC063）
