现代食品科技2017，Vol.33Issue(6)：32-37,6.DOI:10.13982/j.mfst.1673-9078.2017.6.006
酪蛋白水解物分别与维生素A、维生素C和维生素E协同保护乙醇氧化损伤的肝细胞
Synergistic Protective Effects of Casein Hydrolysate and Vitamins A, C, or E against Ethanol-induced Damage in Hepatocyte HHL-5 Cells
摘要
Abstract
Casein hydrolysate (CH) can be used to treat and repair hepatocytes damaged by ethanol-induced oxidative stress.The aim of this study was to evaluate the synergistic effects of vitamin A,vitamin C,or vitamin E with CH to protect the liver cells from oxidative damage in vitro.Previous studies have shown that CH has no obvious toxicity to liver cells (HHL-5),and can even promote proliferation.The results showed that 300 mmol/L ethanol induced obviously oxidative damage to HHL-5 cells,and CH significantly enhanced the viability of these cells.Combining vitamin A (0.0344 mg/mL),vitamin C (0.0881 mg/mL),or vitamin E (0.1077 mg/mL) with CH significantly enhanced the viability of liver cells damaged by ethanol-induced oxidation.The concentration of CH for the optimum synergistic effect was selected using a cell counting kit-8 (CCK-8) assay and determination of lactate dehydrogenase (LDH) content in the culture medium.This concentration was verified through the determination of intracellular content of malondialdehyde (MDA) and reactive oxygen species (ROS).CH at 2 mg/mL alone exhibited a certain restorative effect on cells damaged by ethanol-induced oxidation,and the synergistic protective effects of combining CH at this concentration with vitamin A,vitamin C,or vitamin E were significantly higher (p＜0.05).关键词
酪蛋白水解物/乙醇/氧化损伤/协同保护Key words
casein hydrolysate/ethanol/oxidative stress/synergistic protection引用本文复制引用
庞佳楠,陈芳芳,马春敏,戚莉佳,李铁晶..酪蛋白水解物分别与维生素A、维生素C和维生素E协同保护乙醇氧化损伤的肝细胞[J].现代食品科技,2017,33(6):32-37,6.基金项目
国家自然科学基金项目(31201337) （31201337）
