现代食品科技2017，Vol.33Issue(6)：38-44,7.DOI:10.13982/j.mfst.1673-9078.2017.6.007
基因表达谱技术分析棒曲霉毒素肾细胞毒性的机制
Mechanisms of Patulin (PAT) Toxicity towards Human Embryonic Kidney (HEK)293 Cells, as Determined by Digital Gene Expression Profile Analysis
摘要
Abstract
The dynamic changes in gene expression profiles of human embryonic kidney (HEK)293 cells during the injury process induced by food contaminant patulin (PAT) were analyzed.HEK293 cells were treated with 5 μM PAT for 3 h and 24 h,and the differentially expressed genes were screened using a digital gene expression (DGE) tag profiling technique.Gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were carried out,and some of the differentially expressed genes were verified by real-time quantitative polymerase chain reaction (PCR).The results showed that 79 and 261 genes were differentially expressed after 3-h and 24-h PAT induction,respectively.Among them,71 and 192 genes were upregulated,while 8 and 69 genes were downregulated.PAT mainly induced the differential expression of apoptosis-related genes;differentially expressed RNA degradation genes were all upregulated,and differentially expressed RNA polymerase genes were downregulated.Most differentially expressed genes of ubiquitin-mediated proteolysis were upregulated.Mitogen-activated protein kinase (MAPK) cascade signaling genes were mainly involved in the downstream process,and most related genes were upregulated.The DGE technique provided numerous clues to renal cell damage induced by PAT at the molecular level and laid a foundation for the study of the biological effects of related genes.关键词
棒曲霉毒素/数字基因表达谱/人胚肾细胞/凋亡Key words
patulin/digital gene expression tag profile/human embryonic kidney cells/apoptosis引用本文复制引用
甘婧,张百刚,师振强,钟玉杰,王晓瑞,晋程妮,夏效东,彭晓丽..基因表达谱技术分析棒曲霉毒素肾细胞毒性的机制[J].现代食品科技,2017,33(6):38-44,7.基金项目
国家自然科学基金资助项目(31571928、31201286) （31571928、31201286）
中央高校基本科研创新一般项目(2014YB021) （2014YB021）
