现代食品科技2017，Vol.33Issue(6)：45-52,8.DOI:10.13982/j.mfst.1673-9078.2017.6.008
酸性黑木耳多糖协同抗凝血作用分析
Synergistic Effect of Acidic Auricularia auricular Polysaccharide on Anticoagulant Activity
摘要
Abstract
To study the synergistic effect of an acidic Auricularia auricular polysaccharide (Ac-AAP) with other polysaccharides on anticoagulant activity,tea polysaccharide (TP),jujube polysaccharide (JP),and seaweed polysaccharide (SP) were combined with Ac-AAP,respectively.The Chou-Talalay combination index (CI),percentage inhibition of conversion of fibrinogen (FIB) to fibrin,activated partial thromboplastin time (APTT),prothrombin time (PT),and thrombin time (TT) were used as indicators to evaluate the synergistic effect of Ac-AAP with other three polysaccharides on anticoagulant activity.The experimental results revealed that the Ac-AAP∶SP (1 +2) combination had the strongest anticoagulant activity (p＜0.05),and the CI value was 0.63175.Comprehensive evaluation of the anticoagulant activity showed that with a polysaccharide concentration of 2.0 mg/mL,values of all anticoagulation indices of Ac-AAP∶SP (1 +2) were better than those of the other combinations (p＜0.05);the percentage inhibition of conversion of FIB to fibrin was 91.06±0.79 ％,the APTT of plasma was 95.47±0.61 s,and the TT value was 62.24a0.53 s,but the PT of plasma was not significantly different from that of saline (negative control).关键词
酸性黑木耳多糖/协同作用/抗凝血活性Key words
acidic Auricularia auricular polysaccharide/synergistic action/anticoagulant activity引用本文复制引用
顾嘉林,康宁,李德海,周聪,杜令娟,符群..酸性黑木耳多糖协同抗凝血作用分析[J].现代食品科技,2017,33(6):45-52,8.基金项目
中央高校基本科研业务费专项资金资助(2572014CA19) （2572014CA19）
黑龙江省自然科学基金面上项目(C2015062) （C2015062）
黑龙江省教育厅科学技术研究项目(12543015) （12543015）
