现代食品科技2017，Vol.33Issue(6)：53-61,99,10.DOI:10.13982/j.mfst.1673-9078.2017.6.009
富硒对黄粉虫蛋白分布及其酶解多肽抗氧化活性的影响
Effects of Selenium Enrichment on Protein Distribution in Tenebrio molitor and Antioxidative Activity of Enzymatically Hydrolyzed Tenebrio molitor Peptides
摘要
Abstract
The effects of selenium-enrichment on Tenebrio molitor (mealworm) protein and its selenium concentration were evaluated.Based on solubility,water-,salt-,ethanol-,and alkali-soluble proteins from T.molitor were extracted,and the selenium concentration was measured using an atomic fluorescence spectrometer.Selenium-enriched mealworm protein was hydrolyzed by enzymes to obtain Se-containing T.molitor peptide (Se-TMP),and the cellular antioxidant activities of Se-TMP and TMP were determined and compared using erythrocyte hemolysis as a model.The results showed that the dry weight ratio ofmealworm proteins after Se-enrichment increased from 53.86％ to 59.12％and electrophoretic bands at 75 and 37 ku were darker in Se-enriched proteins.The total selenium concentration in Se-enriched T.molitor was 573.32 μg/kg and organic selenium concentration was 524.91 μg/kg (91.56％).In soluble protein,selenium was mainly present in alkali-soluble protein,accounting for 26.62％ of the total selenium.Furthermore,46.63％ of total selenium was found in the insoluble component.At a sample concentration of 3 mg/mL,the inhibition rate of Se-TMP on erahroeyte hemolysis was 87.32％.The inhibition rates of Se-TMP were all higher than those of TPM at various sample concentrations.关键词
黄粉虫/硒/蛋白/多肽/抗氧化Key words
Tenebrio molitor, selenium/protein/peptides/antioxidant activity引用本文复制引用
林晏因,闵甜,赖富饶,张晓元,吴晖..富硒对黄粉虫蛋白分布及其酶解多肽抗氧化活性的影响[J].现代食品科技,2017,33(6):53-61,99,10.基金项目
广东省科技项目(2016A040402020) （2016A040402020）
广东省科技项目(2016B010121014) （2016B010121014）
韶关市科技项目(2014CX/N310) （2014CX/N310）
