现代食品科技2017，Vol.33Issue(6)：118-124,79,8.DOI:10.13982/j.mfst.1673-9078.2017.6.018
甜叶菊毛状根培养系统的建立及绿原酸类成分的诱导
Establishment of Stevia rebaudiana Bertoni Hairy Root Culture System and Induction of Chlorogenic Acids
摘要
Abstract
Stevia rebaudiana hairy roots were induced using Agrobacterium rhizogenes A4.A culture system to produce chlorogenic acids in hairy roots was then established,and the effects ofmethyljasmonate and salicylic acid on the accumulation ofchlorogenic acids in hairy roots were studied.The results showed that Stevia rebaudiana hairy roots were successfully reproduced after 14 days' culture of Stevia rebaudiana leaf explants infected with Agrobacterium rhizogenes A4.Polymerase chain reaction (PCR) results indicated that genes rolB and rolC from Agrobacterium rhizogenes were integrated into the genome of Stevia rebaudiana.Hairy roots were cultured in Murashige and Skoog (MS)liquid medium for 14 d,and various concentrations (50,100,and 200 μmol/L) of methyl jasmonate and salicylic acid were added to the cultures,respectively.After 1 d,3d,and 6 d treatments,both plant elicitors inhibited the growth of hairy roots.However,methyl jasmonate promoted the accumulation of chlorogenic acids in hairy roots,and salicylic acid inhibited the accumulation of chlorogenic acids.Thus,Stevia rebaudiana hairy root was induced by Agrobacterium rhizogenes A4 and could be used to produce chlorogenic acids;this could be promoted by methyl jasmonate elicitor.关键词
发根农杆菌/毛状根/茉莉酸甲酯/水杨酸/绿原酸类Key words
Agrobacterium rhizogenes/hairy root/methyl jasmonate/salicylic acid/chlorogenic acids引用本文复制引用
邹凯,刘泽波,许小向,上官新晨,尹忠平,陈继光,蒋艳..甜叶菊毛状根培养系统的建立及绿原酸类成分的诱导[J].现代食品科技,2017,33(6):118-124,79,8.基金项目
赣鄱英才555工程 （）
江西高校科技落地计划项目(KJLD12021) （KJLD12021）
江西省食品药品监督管理局科技计划(2015yp17) （2015yp17）
江西省科技支撑计划项目(2010BNB00503) （2010BNB00503）
