现代食品科技2017，Vol.33Issue(6)：150-157,8.DOI:10.13982/j.mfst.1673-9078.2017.6.022
红曲发酵液中水溶性红曲黄色素的吸附分离
Adsorption and Separation of Monascus Yellow Pigments in Monascus Fermentation Broth
摘要
Abstract
The adsorption and isolation capacities of macroporoas resins with different polarities,ion exchange resins,activated carbon,and silica gel on water-soluble yellow pigments of submerged Monascus fermentation broths were studied.The results showed that nonpolar macroporous resin DA201-C exhibited the best performance;the adsorption isotherm of DA201-C was well fitted with the Freundlich equation,and the adsorption kinetics followed thepseudo-first-order kinetics model,indicating that adsorption was not restricted to monolayer adsorption.Liquid film diffusion was the major rate-limiting step,and the diffusion rate constant was 0.16 min-1.The purification conditions were optimized by static and dynamic adsorption and desorption experiments,and the results were analyzed using SPSS software.The optimal desorption performance was achieved when the solid material-to-liquid ratio of static adsorption and desorption was 1∶2,both the adsorption and desorption times were 15 min,the eluent was 100％ ethanol,and the eluent was acidic.When the flow rate of dynamic adsorption and desorption was 0.25BV/min,the water-soluble Monascus yellow pigments could be obtained with high recovery (96.99％),desalination (99.44％),and desugarization rate (92.52％).The preliminary scale-up experiment showed a stable adsorption and desorption efficiency.In conclusion,the method using DA201-C resin to isolate and purify the water-soluble Monascus yellow pigments in fermentation liquid is easy to operate,has a short cycle and good performance in a scale-up experiment,and shows a great application value in industrial production.关键词
吸附动力学/水溶性红曲黄色素/树脂/脱盐/脱糖Key words
adsorption kinetics/water-soluble Monascus yellow pigments/resin/desalination/desugarization引用本文复制引用
吴振强,汪梅花,唐锐,陈功..红曲发酵液中水溶性红曲黄色素的吸附分离[J].现代食品科技,2017,33(6):150-157,8.基金项目
国家自然科学基金项目(31271925) （31271925）
广东省教育部产学研结合项目(2013B090600015) （2013B090600015）
广州市科技计划项目(2014J4100192) （2014J4100192）
