现代食品科技2017，Vol.33Issue(6)：326-331,6.DOI:10.13982/j.mfst.1673-9078.2017.6.048
呋喃唑酮代谢物单克隆抗体和胶体金免疫层析试纸条的研制
Preparation of Monoclonal Antibody and Colloidal Gold Immunochromatographic Test Strip against Furazolidone Metabolites
摘要
Abstract
The aim of the study was to establish a method for the rapid detection of the furazolidone metabolites.A conjugate of the derivatives of furazolidone metabolites and bovine serum albumin (BSA) was used to immunize mice.The cells were prepared by monoclonal antibody technology,and the positive clones were screened by indirect ELISA and icELISA.Monoclonal antibodies purified from ascitic fluid were used in the colloidal gold tag and for thepreparation of the colloidal gold immunochromatographic test strip.Two cell types were obtained,which could stably secrete antibodies.The titer of the purified antibody named 4G3 was 1∶106,and the median inhibitory concentration (IC50)was 1.7 μg/L with affinity constant Ka=1.6× l09 L/mol.The results showed that the detection using the test strips was limited to four μg,/L,there were no cross reactions between CPAHD,CPSEM,and CPAMOZ,and the test results of the samples were consistent with the results of HPLC.In this study,specific monoclonal antibodies against CPAOZ were prepared,and colloidal gold immunochromatographic test strips were developed based on the antibodies,which could achieve rapid and sensitive detection of furazolidone metabolite residues.关键词
呋喃唑酮代谢物/单克隆抗体/胶体金免疫层析试纸条Key words
furazolidone metabolites/monoclonal antibody/colloidal gold immunochromatographic test paper引用本文复制引用
李春生,刘静静,杜顺丰,武孝丽,曹秀梅,吴萌,张娜..呋喃唑酮代谢物单克隆抗体和胶体金免疫层析试纸条的研制[J].现代食品科技,2017,33(6):326-331,6.基金项目
河北省科技支撑计划项目(15273205D) （15273205D）
