食品科学2017，Vol.38Issue(11)：1-5,5.DOI:10.7506/spkx1002-6630-201711001
牛乳α-乳白蛋白IgE线性表位的关键氨基酸识别
Identification of the Critical Amino Acids of IgE-Binding Epitopes in α-Lactalbumin
摘要
Abstract
α-Lactalbumin represents one of the major allergens causing cow milk allergy.The identification of the epitopes of α-lactalbumin and the critical amino acids for its allergenicity is of great significance for understanding the mechanism of action of α-lactalbumin and developing hypoallergenic dairy products.In this study,a series of peptides were synthesized by a solid phase method for the characterization of immunological epitopes and critical amino acids.The immunoglobulin (Ig) E-binding epitopes were immunolabeled with individual sera from cow milk-allergic patients as probes by enzyme linked immunosorbent assay (ELISA).Alanine scanning of immunodominant epitopes was used to identify the critical amino acids (aa).The results showed that IgE-binding epitopes were located within the sequences of aa1-15,aa6-20,aa46-60,aa71-85 and aa101-115.Our initial data revealed that Val8,Phe9,Arg10,Tyr103,Leu105 and His107 were the critical amino acids for IgE-binding epitopes.This study will provide the necessary information to alter the cDNA to encode a protein capable of activating milk-specific T cells,but with reduced IgE-binding capacity.关键词
牛乳过敏/α-乳白蛋白/作用表位/关键氨基酸Key words
cow milk allergy/α-lactalbumin/epitope/critical amino acid分类
轻工纺织引用本文复制引用
丛艳君,陈澍,李晔,于晓凤,李林峰..牛乳α-乳白蛋白IgE线性表位的关键氨基酸识别[J].食品科学,2017,38(11):1-5,5.基金项目
国家自然科学基金青年科学基金项目(31101236) （31101236）
北京市自然科学基金面上项目(6132004) （6132004）
北京市科技新星计划项目(Z131102000413005) （Z131102000413005）
