食品科学2017，Vol.38Issue(11)：52-58,7.DOI:10.7506/spkx1002-6630-201711009
不同部位鲽鱼皮酸溶性胶原蛋白的提取及其特性
Extraction and Characterization of Collagens from Different Skins of Flounder (Pleuronichthys cornutus)
摘要
Abstract
In this study,acid-soluble collagens were extracted from black and white skins of fresh flounder,and their physicochemical characteristics,emulsifying activity and emulsion stability were compared and analyzed.The structural characteristics were studied by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM).Additionally,solubility,rheological properties,isoelectdc point,emulsifying activity index and emulsion stability were also investigated.FTIR analysis proved the existence of amide A,amide B,amide Ⅰ,amide Ⅱ,and amide Ⅲ,confirming that the triple helical chain conformation of the two collagens was completely maintained.The SEM results indicated that both collagens showed a porous,evenly distributed network structure,which proved that the original structure of collagen fibers was basically maintained during the extraction process.The emulsifying activity index (EAI) of the black skin collagen was significantly lower than that of the white skin collagen (P ＜ 0.05),which were (2.60 ± 0.53) and (7.30 ± 0.41) m2/g,respectively,while the emulsion stability index (ESI) of the former was significantly higher than that of the latter (P ＜ 0.05),which were (49.12 ± 5.18) and (27.56 ± 4.91) min,respectively.These results showed that certain differences exist in physicochemical characteristics and functional properties of acid-soluble collagens from different flounder skins.关键词
鲽鱼/鱼皮/酸溶性胶原蛋白/提取/理化特性Key words
Pleuronichthys cornutus/fish skin/acid-soluble collagen/extraction/physicochemical characterization分类
轻工纺织引用本文复制引用
蔡路昀,马帅,曹爱玲,李秀霞,张宇昊,赵国华,赵元晖,林洪,励建荣..不同部位鲽鱼皮酸溶性胶原蛋白的提取及其特性[J].食品科学,2017,38(11):52-58,7.基金项目
国家自然科学基金青年科学基金项目(31401478) （31401478）
中国博士后基金面上项目(2015M570760) （2015M570760）
“十二五”国家科技支撑计划项目(2012BAD29B06) （2012BAD29B06）
辽宁省自然科学基金项目(20170540006) （20170540006）
重庆市博士后特别资助项目(Xm2015021) （Xm2015021）
渤海大学研究生创新基金项目(071502224-48) （071502224-48）
