食品科学2017，Vol.38Issue(11)：214-219,6.DOI:10.7506/spkx1002-6630-201711034
Caco-2细胞模型用于乳源ACE抑制肽LL、LPEW的小肠吸收研究
Intestinal Absorption of Milk-Derived ACE Inhibitory Peptides LL and LPEW Using Caco-2 Cell Model
摘要
Abstract
Caco-2 cell model was established and verified to analyze the transport capacity of ACE inhibitory peptides LL and LPEW and to explore their transport mechanism across intestinal epithelial cells.Morphology,transepithelial electrical resistance and alkaline phosphatase activity were measured to verify the validity of the Caco-2 cell model.Apparent permeability coefficient (Papp) of LL and LPEW were (275.17 + 8.28) × 10-7 and (5.13 ± 1.49) × 10-7cm/s,respectively in Caco-2 transport experiments,suggesting that LL displayed much better intestinal absorption than LPEW.The transportation route of LL may be endocytosis,as demonstrated by comparing the transport capacity of LL with and without transport inhibitors including the paracellular transport accelerator sodium deoxycholate,the endocytosis inhibitor Wortmannin,and the competitive inhibitor of the peptide transports Gly-Pro.LL had no efflux activity,as suggested by comparing the transport capacity with and without efflux inhibitor including the P-glycoprotein inhibitor verapamil,the multidrug resistance protein inhibitor MK-571,and the ATPase inhibitor sodium azide.In conclusion,LL showed good intestinal absorption.关键词
ACE抑制肽/Caco-2细胞模型/小肠吸收Key words
angiotensin-Ⅰ converting enzyme (ACE) inhibitory peptides/Caco-2 cell model/intestinal absorption分类
轻工纺织引用本文复制引用
高锦锦,郭宇星,潘道东..Caco-2细胞模型用于乳源ACE抑制肽LL、LPEW的小肠吸收研究[J].食品科学,2017,38(11):214-219,6.基金项目
江苏省自然科学基金项目(BK20151544 （）
BK20141447) （）
国家自然科学基金面上项目(31571852 （）
31471598) （）
