食品科学2017，Vol.38Issue(11)：220-224,5.DOI:10.7506/spkx1002-6630-201711035
干酪乳杆菌对HepG2细胞抗氧化功能的影响
Effect of Lactobacillus casei on Antioxidant Function of HepG2 Cells
摘要
Abstract
Objective:This experiment was conducted to study the effect of Lactobacillus casei on antioxidant function of HepG2 cells under oxidative stress.Methods:HepG2 cells were randomly divided into 3 groups:control,model (H2O2 induced oxidative stress) and treatment (H2O2 plus Lactobacillus casei) groups.Antioxidant activity in culture supernatants and lysates of HepG2 cells at 12 and 48 h were measured.4',6-diamidino-2-phenylindole (DAPI) staining was applied to observe cell morphology in different groups by fluorescence microscopy.Results:Total antioxidant capacity (T-AOC),and glutathione peroxidase (GSH-Px) and catalase (CAT) activities in the culture supernatant,as well as superoxide dismutase (SOD) activity in the cell lysate at 12 h in the treatment group significantly increased by 80％,30％,124％ and 22％,respectively when compared with their counterparts in the model group (P ＜ 0.05).At 24 h,the activities of T-AOC,SOD,GSH-Px,CAT and peroxidase (POD) were significantly increased (P ＜ 0.05),and the content of glutathione (GSH) and malondialdehyde (MDA) in the culture supernatant in the treatment group significantly decreased by 29％ and 63％ (P ＜ 0.05);a significant decrease of 20％ in SOD activity in the cell lysate was observed for the treatment group compared with the model group (P ＜ 0.05).Moreover,the number of damaged cells was reduced in the treatment group than that in the model group,and most cells in the former group had normal morphology.Conclusion:The addition of Lactobacillus casei could increase antioxidant function of HepG2 cells under oxidative stress.关键词
干酪乳杆菌/HepG2细胞/抗氧化/氧化应激/细胞裂解液Key words
Lactobacillus casei/HepG2 cells/antioxidant/oxidative stress/cell lysates分类
轻工纺织引用本文复制引用
陈佩,党辉..干酪乳杆菌对HepG2细胞抗氧化功能的影响[J].食品科学,2017,38(11):220-224,5.基金项目
陕西省农业科技创新与攻关项目(2015NY025) （2015NY025）
陕西广播电视大学重点课题(15D-08-A02) （15D-08-A02）
