食品科学2017，Vol.38Issue(12)：57-62,6.DOI:10.7506/spkx1002-6630-201712009
补料方式对灵芝菌丝体液态深层发酵合成灵芝三萜的影响
Effects of Different Feeding Methods on Production of Triterpenes by Ganoderma lucidum in Submerged Fermentation
摘要
Abstract
Four different feeding methods for the production of triterpenes by submerged fed-batch fermentation of Ganoderma lucidum G0119 in a 50 L-fermentor were compared.The results showed that glucose feeding during fermentation could obviously promote the mycelical growth of G.lucidum G0119 and the synthesis of triterpenes,and different feeding methods had different influences on the mycelial dry weight and the concentration of triterpenes in the fermentation broth.The highest mycelical dry weight could be obtained by exponential feeding,as well as high concentration of triterpenes.The results showed that the feeding strategy was the best among all tested ones,giving a myeelial dry weight of 17.68 g/L and a triterpene content of 4.58 g/100 g dry mycelium at the end of fermentation,which were significantly increased by 65.70％ and 100.88％,respectively,as compared with fed-batch fermentation.关键词
灵芝/深层发酵/灵芝三萜/补料培养Key words
Ganoderma lucidum/submerged culture/triterpenes/fed-batch culture分类
化学化工引用本文复制引用
冯杰,冯娜,唐庆九,颜梦秋,杨焱,周帅,刘艳芳,刘方,张劲松..补料方式对灵芝菌丝体液态深层发酵合成灵芝三萜的影响[J].食品科学,2017,38(12):57-62,6.基金项目
上海市市级农口系统青年人才成长计划资助项目(沪农青字2015第1-7号) （沪农青字2015第1-7号）
上海市农业科学院青年科技人员“助跑”计划项目(ZP06) （ZP06）
