食品科学2017，Vol.38Issue(12)：63-68,6.DOI:10.7506/spkx1002-6630-201712010
高糖胁迫下槲皮素对酿酒酵母胞内损伤的保护作用与机制
Protective Effect and Mechanism of Quercetin against High Glucose-Induced Intracellular Damage in Saccharomyces cerevisiae
摘要
Abstract
In this study,the protective effect of quercetin on high glucose-induced damage was investigated in Saccharomyces cerevisiae.Compared with the control group,the activities of superoxide dismutase (SOD),catalase (CAT),and peroxidase (POD) were all significantly decreased under high glucose-induced stress (P ＜ 0.05),but intracellular ROS was no obviously changed.After being treated with quercetin,the ROS levels and SOD and CAT activities were dramatically inhibited (P ＜ 0.05),but the POD activity was significantly enhanced in comparison to the control and high glucose groups (P ＜ 0.01).The results showed that POD activity was more sensitive than other indicators in reflecting the resistance to high glucose-induced stress,and quercetin could effectively enhance the antioxidant capacity by regulating POD activity in S.cerevisiae S288c.In addition,high glucose-induced stress resulted in an significant decrease in the expression levels of glycerol-3-phosphate dehydrogenase 2 (GPD2) and sucrose transport protein-encoding genes (SUC2) (P ＜ 0.05),but a significant increase in the expression level of hexose transport-1-encoding gene (HXT1) (P ＜ 0.01),compared with the control.After being treated with quercetin,the expression profiles of GPD2,SUC2,and HXT1 were significantly increased in S.cerevisiae S288c (P ＜ 0.05).However,the expression level of glycerol kinase gene (GUT1) was not changed significantly.These results suggested that quercetin could reduce high glucose-induced damage by regulating the high osmolarity glycerol (HOG) signaling pathway,inulin catabolism pathway and hexose transport pathway to accelerate glucose decomposition.Therefore,we conclude that quercetin plays an important role in protecting S.cerevisiae from damage caused by high glucose-induced stress,and the underlying mechanism may be associated with its antioxidant effect and being involved in the HOG signaling pathway and the glucose decomposition and transport pathways in S.cerevisiae.关键词
酿酒酵母/高糖胁迫/槲皮素/酶比活力/基因表达水平Key words
Saccharomyces cerevisiae/high glucose-induced stress/quercetin/enzyme activitity/expression levels分类
轻工纺织引用本文复制引用
徐晓丹,潘宇,柯尊丽,周志钦..高糖胁迫下槲皮素对酿酒酵母胞内损伤的保护作用与机制[J].食品科学,2017,38(12):63-68,6.基金项目
重庆市基础与前沿研究计划项目(cstc2014jcyjA80026) （cstc2014jcyjA80026）
2016食用农产品特征性营养组成识别与验证评估专项项目(GJFP201601501 （）
GJFP201601503) （）
重庆市现代特色效益农业技术体系创新项目(20164-4) （20164-4）
