食品科学2017，Vol.38Issue(12)：125-130,6.DOI:10.7506/spkx1002-6630-201712019
‘紫娟’茶花色苷的分离鉴定
Separation and Identification of Anthocyanins from ‘Zijuan’ Tea Leaves
摘要
Abstract
The anthocyanins from leaves of the tea cultivar ‘Zijuan’ were separated and purified by sequential column chromatography using MCI gel CHP 20P resin (75-150 μm) and SephadexTM LH-20,through gradient elution with 5％ acidmethanol and 5％ acid-water.A total of 6 anthocyanins were separated from the purified extract,which were analyzed by high performance liquid chromatography (HPLC),thin layer chromatography (TLC) and HPLC-electrospray ionizationtandem mass spectrometry (HPLC-ESI-MS/MS).The results showed that the anthocyanins were identified as delphinidin 3-O-β-D-galactoside,cyanidin 3-O-β-D-galactoside,delphinidin 3-O-β-D-(6-(Z)-p-coumaroyl)galactopyranoside,cyanidin 3-O-β-D-(6-(Z)-p-coumaroyl)galactopyranoside,delphinidin 3-O-β-D-(6-(E)-p-coumaroyl)galactopyranoside and cyanidin 3-O-β-D-(6-(E)-p-coumaroyl)galactop yranoside.关键词
‘紫娟’茶/花色苷/分离纯化/高效液相色谱-电喷雾-串联质谱法/结构鉴定Key words
‘Zijuan’ tea/anthocyanin/separation and purification/high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS)/structure identification分类
轻工纺织引用本文复制引用
李燕丽,罗琼仙,杨雪梅,占琪,李家华..‘紫娟’茶花色苷的分离鉴定[J].食品科学,2017,38(12):125-130,6.基金项目
国家自然科学基金地区科学基金项目(31360196) （31360196）
