食品科学2017，Vol.38Issue(12)：164-170,7.DOI:10.7506/spkx1002-6630-201712025
霍山铁皮石斛多糖的脱蛋白工艺及结构分析
Deproteinization and Structural Analysis of Polysaccharides from Dendrobium officinale Kimura et Migo Grown in Huoshan
摘要
Abstract
In this study,the deproteinization and structural characterization of the polysaccharides extracted from Dendrobium officinale Kimura et Migo grown in Huoshan county of Anhui province were investigated.Three protein removal methods:the Sevage method,enzymatic hydrolysis and their combination were comparatively evaluated in terms of protein removal and polysaccharide loss rates.The combined method was found to have the highest deproteinization efficiency,yielding 81.58％ protein removal and 15.63％ polysaccharide loss.After purification of the polysaccharides by successive DEAE-cellulose-52 and Sephadex G-100 column chromatography,an active fraction named as DOPA-1 was obtained.By using UV spectroscopy and high performance liquid chromatography (HPLC),the polysaccharide DOPA-1 was confirmed to be homogeneous.Moreover,DOPA-1 was mainly composed of mannose,glucose and galactose as revealed by GC analysis,with a molar ratio of 1:0.42:0.27.IR spectroscopy and Cango red test indicated that DOPA-1 contained β-D-mannopyranose and possessed a triple-helix conformation.关键词
铁皮石斛多糖/脱蛋白/分离纯化/结构分析Key words
Dendrobium officinale polysaccharide/removal of protein/purification/structural analysis分类
医药卫生引用本文复制引用
王琳炜,欧阳臻,张碧娟,杨倩,王笃军,于一凡,张杰,马青..霍山铁皮石斛多糖的脱蛋白工艺及结构分析[J].食品科学,2017,38(12):164-170,7.基金项目
国家自然科学基金面上项目(81573529 （）
81072985 （）
81373480) （）
中央本级重大增减支项目(2060302) （2060302）
安徽省石斛产业化开发协同创新项目 （）
江苏大学第14批大学生科研立项项目(14A134) （14A134）
