食品科学2017，Vol.38Issue(12)：217-222,6.DOI:10.7506/spkx1002-6630-201712033
一种基于多重实时荧光聚合酶链式反应熔解曲线分析的肉及肉制品掺假鉴别方法
A Multiplex RT-PCR Melting Curve Analysis Method for Adulteration Identification in Meat and Meat Products
摘要
Abstract
This study was aimed to establish a rapid method for the identification of adulterated meat and meat products.Five pairs of species-specific primers,yielding amplicons having significantly different melting temperatures (Tm),were designed targeting the mitochondrial COXⅠ gene of pig and bovine,the 16S rRNA gene of sheep,goat,dog,fox and raccoon,and the 12S rRNA gene of chicken and duck,respectively.The established multiplex RT-PCR-melting curve analysis method was evaluated in term of specificity and sensitivity and was applied in the detection of commercial meat products.The method showed a good specificity and sensitivity,and was able to detect 0.001-1 ng of DNA from single species and 0.1 ng of DNA form mixed species.Thus,it can be used to test both raw and processed meat products.关键词
肉类掺假/多重实时荧光聚合酶链式反应/熔解曲线分析/SYBR Green染料/动物源性成分Key words
meat adulteration/multiplex real-time PCR/melting curve analysis/SYBR Green dye/animal-derived ingredient分类
轻工纺织引用本文复制引用
周彤,李家鹏,李金春,乔晓玲,黄鑫,陈曦,杨君娜,郭雅..一种基于多重实时荧光聚合酶链式反应熔解曲线分析的肉及肉制品掺假鉴别方法[J].食品科学,2017,38(12):217-222,6.基金项目
国家自然科学基金面上项目(31271877) （31271877）
“十二五”国家科技支撑计划项目(2014BAD04B05) （2014BAD04B05）
