食品科学2017，Vol.38Issue(12)：273-277,5.DOI:10.7506/spkx1002-6630-201712042
乳胶免疫层析法定量检测猪尿液中的盐酸克伦特罗
Development of Dyed Latex Bead-Based Immunochromatographic Assay for Quantitative Detection of Clenbuterol in Swine Urine
摘要
Abstract
Objective:Aiming at the development of an immunochromatographic method based on dyed latex beads for the detection of clenbuterol (CLB) in swine urine.Methods:Dyed latex beads were conjugated with anti-CLB antibody and vacuum freeze-dried.CLB-bovine serum albumin (CLB-BSA) conjugate and goat anti-mouse IgG antibodies were coated onto nitrocellulose membranes to establish test and control lines,respectively.The test was carried out by placing swine urine into the sample well and mixed with dyed latex beads.After incubation,the optical density was measured by a microplate reader.Results:After optimizing the physical and chemical parameters,the best surfacant was Tween-20,300 nm dyed latex beads were chosen,and the incubation time was 9 minutes.The limit of detection (LOD) was 0.013 ng/mL.The recoveries from spiked swine urine varied from 97.8％ to 106.0％,with relative standard deviation of less than 9.6％.Conclusion:A sensitive and accurate method for the detection of CLB was successfully developed in this paper.关键词
免疫层析试纸/彩色乳胶/盐酸克伦特罗/猪尿液/定量检测Key words
immunochromatographic strip/dyed latex beads/clenbuterol/swine urine/quantitative detection分类
轻工纺织引用本文复制引用
管笛,魏颖,王旗,李俊博,武会娟..乳胶免疫层析法定量检测猪尿液中的盐酸克伦特罗[J].食品科学,2017,38(12):273-277,5.基金项目
北京市优秀人才项目(2014400685627G277) （2014400685627G277）
北京市科学技术研究院青年骨干计划项目(201415) （201415）
