食品科学2017，Vol.38Issue(12)：292-297,6.DOI:10.7506/spkx1002-6630-201712045
用于农残快速检测的离心式微流控芯片研制
Fabrication and Application of Centrifugal Microfludic Chip for Rapid Detection of Pesticide Residues
摘要
Abstract
In this paper,a novel disposable centrifugal microfludic chip with pre-storage biochemical reagents was developed for the rapid detection of pesticide residues in agricultural products,which was based on the principle of enzyme inhibition and photometric analysis.The microfludic chip was integrated with sampling,enzyme inhibition,color development and detection chamber.By coupling with a portable detection device developed in our laboratory,the microfludic chip could provide a simple,easy-to-use,low-cost and sensitive approach for rapid detection of organophosphate and carbamate pesticides on site.Results indicated that the microfluidic chip could achieve an automatic detection process with the advantages of less consumption of reagents (reduction by one-twentieth) and satisfied accuracy compared with the traditional pesticide residue detector.Furthermore,the microfluidic chip was proved to be a powerful tool for screening pesticide residues in the case of high-throughput requirement.The microfluidic chip was superior to the traditional pesticide residue detector in term of detection sensitivity,reproducibility and measurement accuracy.The microfluidic chip coupled with self-made detection device is particularly suitable for on-site,rapid and high-throughput screening of pesticide residues by nonprofessionals.关键词
微流控芯片/农药残留/快速检测/酶抑制法Key words
microfluidic chip/pesticide residues/rapid detection/acetylcholin esterase (Ache) inhibition method分类
轻工纺织引用本文复制引用
叶嘉明,邵佳美,杨平,范真真,冯何凯,陈波,杜汉根..用于农残快速检测的离心式微流控芯片研制[J].食品科学,2017,38(12):292-297,6.基金项目
食品科学与工程浙江省重中之重一级学科开放基金项目(JYTSP20142062) （JYTSP20142062）
嘉兴市公益性应用技术研究计划项目(2015AY23006) （2015AY23006）
