中国食品添加剂Issue(6)：57-64,8.
响应面优化盐析萃取万寿菊花中叶黄素的研究
Study on the optimization of salting-out extraction of lutein from marigold flower by response surface analysis
摘要
Abstract
Salting-out extraction system composed of acetone/K2HPO4 was used to separate lutein from marigold flower.Salting out to determine the optimal system consists of 27％ acetone (w/w) /18％ (w/w) K2HPO4.The effects of three factors on the extraction rate of lutein in marigold flower were studied by response surface methodology.The optimal parameters of three factors were obtained,namely,the quality of dipotassium hydrogen phosphate concentration was 20％,the ratio of material to liquid was 1 ∶ 40,and the extraction temperature was 42 ℃.Under these conditions,the lutein was enriched in the phase of acetone phase,the yield was 6.23 mg/g.The antioxidant activity of marigold flower extracts were assayed with radical-scavenging ability (IC50) of 2,2-diphenyl-1-picrylhydrazyl at room temperature.The marigold flower extracts by salting-out extraction showed lower IC50 (0.34mg/mL) than that by soxhlet and ultrasound-assisted extraction.关键词
盐析萃取/万寿菊/叶黄素/丙酮/磷酸氢二钾/响应面Key words
salting-out extraction/lutein/marigold/acetone/dipotassium hydrogen phosphate/response surface分类
轻工纺织引用本文复制引用
马娜,孙伟鹏,包玲,党艳艳..响应面优化盐析萃取万寿菊花中叶黄素的研究[J].中国食品添加剂,2017,(6):57-64,8.基金项目
国家自然科学基金项目(21266028),新疆兵团高新领域项目(2015AB007),石河子大学高层次人才(RCZX201413). （21266028）
