液相色谱-串联质谱生物分析方法的基质效应和对策OA北大核心CSCDCSTPCD
Matrix Effects and Countermeasure of Liquid Chromatography-Tandem Mass Spectrometry in Bioanalysis
液相色谱-串联质谱(LC-MS/MS)法具有高灵敏度、高选择性、高通量等特点,已经成为生物分析的主流方法,广泛应用于新药发现和开发过程中新化学实体及其代谢物的定量分析.然而,由于生物样品基质成分复杂,共洗脱物质会影响分析物的离子化,使分析物的质谱响应增加或降低,从而影响LC-MS/MS分析方法的准确度和精密度.一般而言,离子抑制较离子增强更为常见.因此,在建立LC-MS/MS法时,需要对离子抑制进行评估和校正,并采用不同的策略消除或减少基质效应的…查看全部>>
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has the characteristics of high sensitivity, high selectivity and high throughput, and has become the mainstream approach for bioanalytical assays.It is widely used for the quantitative analysis of new drug entities (NCEs) and their metabolites in the discovery and development of new drugs.However, due to the complex composition of the biological sample matrix, the co-eluting material affects t…查看全部>>
刘晓云;陈笑艳;钟大放
中国科学院上海药物研究所药物代谢研究中心,上海 201203中国科学院上海药物研究所药物代谢研究中心,上海 201203中国科学院上海药物研究所药物代谢研究中心,上海 201203
化学化工
液相色谱-串联质谱(LC-MS/MS)药动学基质效应
liquid chromatography-tandem mass spectrometry (LC-MS/MS)pharmacokineticsmatrix effect
《质谱学报》 2017 (4)
雷腾舒代谢产物合成及衍生物类药性质研究
388-399,12
国家自然科学基金(81373479, 81521005)资助
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