食品与发酵工业2017，Vol.43Issue(6)：21-26,6.DOI:10.13995/j.cnki.11-1802/ts.201706004
基于在线参数动态调控的双酶法生产葡萄糖酸钠新工艺
A novel approach for sodium gluconate production using two-enzyme method by on-line parameter monitoring and dynamic regulation
摘要
Abstract
In this paper,much attention was paid on the on-line parameters oxygen uptake rate (OUR) and dissolved oxygen (DO) monitoring and control.Based on the changing trends of these parameters,a novel approach of sodium gluconate production was proposed by stepwise addition of glucose oxidase solution dynamically,and the activity of glucose oxidase was kept at an unlimited level during the whole production period.The results showed that in the 50 L reactor with 330 g/L initial glucose concentration,the production time was shortened form 17.0 h to 11.6 h,the yield was enhanced from 1.167 g/g to 1.176 g/g and the overall dosage of used glucose oxidase declined from 2 100 U/g glucose to 1 444 U/g glucose respectively.Compared to the biological fermentation,the novel direct enzymatic reaction displayed significant advantages.The production time was cut down by 42.85％ and the yield was 4.91％ higher than the traditional biological fermentation.The improved direct enzymatic production had more potential value in the further industrial application.关键词
葡萄糖氧化酶/葡萄糖酸钠/在线参数/工艺优化Key words
glucose oxidase/sodium gluconate/on-line parameters/process optimization引用本文复制引用
苏立宇,赵伟,杭海峰,储炬..基于在线参数动态调控的双酶法生产葡萄糖酸钠新工艺[J].食品与发酵工业,2017,43(6):21-26,6.基金项目
国家重点基础研究发展计划(2013CB733600) （2013CB733600）
国家高技术研究发展计划(2015AA021005) （2015AA021005）
