食品与发酵工业2017，Vol.43Issue(6)：49-53,5.DOI:10.13995/j.cnki.11-1802/ts.201706008
环糊精葡萄糖基转移酶生产α-熊果苷的反应条件优化及分子改造
Optimization of conditions for production of α-arbutin by cyclodextrin glucosyltransferase and its molecular modification
摘要
Abstract
α-CGTase of Paenibacillus macerans,β3-CGTase of Bacillus circulans 251,α/β-CGTase of Bacillus stearothermophilus NO2 and Anaerobranca gottschalkii were selected to study the effects of cyclodextrin glucosyltransferase (CGTase) on production of α-arbutin.α-arbutin was synthesized by a two-step enzymatic reaction system of CGTase and amyloglucosidase.The optimum conditions for the synthesis of α-arbutin were optimized,and the highest conversion rate of hydroquinone (HQ) was 25％ by CGTase from Anaerobranca gottschalkii.The optimum conditions for enzyme conversion were as follows:50 g/L maltodextrin with glucose equivalent (DE) of 9％-13％,8 g/L hydroquinone,buffer pH 6.0,reaction at 40 ℃ for 24 h.The production of α-arbutin was determined by high performance liquid chromatography.In order to further improve the conversion rate of HQ,site-directed mutagenesis on CGTase under the optimal reaction conditions was carried out,and the hydroquinone conversion rate of the mutant Y299A was 40％.关键词
环糊精葡萄糖基转移酶/α-熊果苷/酶催化/定点突变Key words
Cyclodextrin glucosyltransferase (CGTase)/α-arbutin/enzyme-catalyzed/site-directed mutagenesis引用本文复制引用
张文蕾,宿玲恰,陶秀梅,吴敬..环糊精葡萄糖基转移酶生产α-熊果苷的反应条件优化及分子改造[J].食品与发酵工业,2017,43(6):49-53,5.基金项目
国家杰出青年基金(31425020) （31425020）
