食品与机械2017，Vol.33Issue(5)：3-7,13,6.DOI:10.13652/j.issn.1003-5788.2017.05.001
降柠檬酸菌株的筛选及鉴定
Study on isolation and identification of reducing citric acid strains
摘要
Abstract
In order to screen the strains which have the capability of reducing the citric acid, 15 strains of yeasts were isolated from the materials of waste water and waste residue.The waste water and residue were collected in the citric acid factory.The yeasts were screened based on the capability of fermenting the citric acid.It was found that the 5 strains had good capability of reducing the citric acid.They could reduce at least 70% citric acid of the culture medium after 5 d fermentation.The 5 strains were tested the stress resistance of ethanol.The strain NS2 and NL2 have strong stress resistance.The morphological and physiology characteristics of NS2 and NL2 were measured by microscopic observation, assimilation of carbon and nitrogen.Then the two strains were identified by 18S rDNA sequencing and the phylogenetic analysis.In the end, NS2 was identified as Pichia kudriavzevii, NL2 was identified as Candida tropicalis.They can be used in the technology of acid reduction in the hawthorn wine in the future.关键词
酵母菌/降酸/毕赤酵母菌/筛选/测序/鉴定Key words
Yeast/Acid Reduction/Pichia/Screen/Sequencing/Identification引用本文复制引用
张铎,毛勇,毛健,刘双平,周志磊..降柠檬酸菌株的筛选及鉴定[J].食品与机械,2017,33(5):3-7,13,6.基金项目
国家自然科学基金-面上项目(编号:31571823) （编号:31571823）
江苏省自然科学基金-面上研究项目(编号: BK20161293) （编号: BK20161293）
2015年度区科技重点研发计划项目(镇江市丹徒区,编号:NY2015009) （镇江市丹徒区,编号:NY2015009）
