食品与机械2017，Vol.33Issue(5)：178-181,4.DOI:10.13652/j.issn.1003-5788.2017.05.036
大孔树脂吸附纯化黔产龙胆草多糖工艺优化
Optimization on adsorption and purification process for gentian polysaccharide in Guizhou by macroporous resin
摘要
Abstract
The content of polysaccharide was determined by phenol sulfuric acid method.The research investigated FL-3 resin on the adsorption and desorption effect of Gentiana scabra Bunge polysaccharide.FL-3 resin was used to purify the polysaccharide.Using the adsorption rate and desorption amount of polysaccharide as the index, the FL-3 resin purification process of polysaccharide was studied.The optimum conditions for the adsorption and resolution of polysaccharide by FL-3 resin were obtained.The experimental results showed that the polysaccharide can be effectively adsorbed and desorbed by the FL-3 resin.The optimum purification conditions were as follows: the sample concentration was 5 mg/mL, the sample flow rate was 2 BV/h, the sample volume was 4 BV, the elution flow rate was about 2 BV/h, and the concentration of ethanol solution was 30%,the elution volume was 200 mL.After purification, the color of polysaccharide become shallow, and the content of that was increased from 26.42% to 71.29%.The recovery rate of polysaccharide was 49.52%.关键词
龙胆草/多糖/纯化/FL-3树脂Key words
Gentiana scabra Bunge/Polysaccharide/Purification/FL-3 macroporous resin引用本文复制引用
宫江宁,饶玉,杨义菊,陈飞..大孔树脂吸附纯化黔产龙胆草多糖工艺优化[J].食品与机械,2017,33(5):178-181,4.基金项目
贵州省科技厅2012年度贵州省科技厅与贵州师范大学联合基金计划项目(编号:黔科合J字LKS[2012]17号) （编号:黔科合J字LKS[2012]17号）
