食品与机械2017，Vol.33Issue(6)：7-12,6.DOI:10.13652/j.issn.1003-5788.2017.06.002
羟基酪醇对LPS引起的小鼠急性肺损伤的保护作用
Protective effect of hydroxytyrosol on LPS-induced acute lung injury in mice
摘要
Abstract
This paper explored the underlying molecular mechanisms by which hydroxytyrosol exerts its anti-inflammatory effects in a murine model of acute lung injury (ALI) by up-regulating autophagy.LPS-induced cytokine activity, inflammatory factor levels, sirtuin (SIRT1/3/6) expression, mitogen-activated protein kinase (MAPK) activation, and autophagy marker expression in ALI-mice were examined by western blotting and staining methods.Molecular docking between HT and SIRT and MAPK was studied with a Sybyl/Surflex module.The results showed that LPS-stimulated SIRT inhibition, MAPK phosphorylation, and autophagy suppression were all notably abolished by HT administration.HT treatment significantly attenuated pulmonary edema and inflammatory cell infiltration into lung tissues, accompanied by decreased lung W/D ratios, protein concentrations, and inflammatory cell levels in BAL fluid.LPS driven release of inflammatory mediators, including TNF-α, IL-1β, IL-6, IL-10, and MCP-1, was strongly regulated by HT.关键词
羟基酪醇/脂多糖/自噬/急性肺损伤/sirtuin蛋白Key words
Hydroxytyrosol/Lipopolysaccharide/Autophagy/acute lung injury/Sirtuin引用本文复制引用
魏静元..羟基酪醇对LPS引起的小鼠急性肺损伤的保护作用[J].食品与机械,2017,33(6):7-12,6.基金项目
辽宁省自然科学基金(编号:20170540480) （编号:20170540480）
