食品与机械2017，Vol.33Issue(6)：13-16,4.DOI:10.13652/j.issn.1003-5788.2017.06.003
脉冲电场对蛋白质与金属离子螯合作用的影响
Study on chelating of metal ion and protein in pulsed electric field
摘要
Abstract
The effects of pulsed electric field on chelating of ovalbumin and metal ions (Cu2+, Ba2+, Mn2+ or Ca2+) and the effect of pulsed electric field on the chelating effect of ovalbumin were studied by Raman spectroscopy.Results: ① PEF treatment time and metal ion species could affect the chelation of metal ions and protein, and the chelation of metal ions with ovalbumin was enhanced with the prolongation of pulse treatment time, when PEF treatment time was 1 695 μs, the chelation of Mn2+ and Cu2+ with protein began to weaken;② The protein and metal ions chelated to different degrees at 1 200~1 700 cm-1, the strongest was Mn2+ , followed by Cu2+ and Ca2+, and the weakest was Ba2+.关键词
脉冲电场/卵清蛋白/拉曼光谱/金属离子/螯合作用Key words
pulsed electric fields/Ovalbumin/Raman spectra/metallic ion/chelation引用本文复制引用
汤婷,刘燕燕,蒋爱民,蒋卓..脉冲电场对蛋白质与金属离子螯合作用的影响[J].食品与机械,2017,33(6):13-16,4.基金项目
国家自然科学基金(编号:21506069) （编号:21506069）
广东省自然科学基金项目(编号:2016A030310455) （编号:2016A030310455）
