现代食品科技2017，Vol.33Issue(7)：1-8,8.DOI:10.13982/j.mfst.1673-9078.2017.7.001
铁皮石斛对缺血再灌注诱导的心律失常的保护作用
Protective Effects of Dendrobium candidum on Ischemia Reperfusion-Induced Arrhythmia
摘要
Abstract
The protective effects and underlying mechanisms ofDendrobium candidum on ischemia reperfusion-induced arrhythmia were explored.Healthy male Sprague Dawley (SD) rats were randomly divided into five groups (n=12),including control group,model group,and three Dendrobium candidum groups,high-dose [1080 mg/(kg·d)],medium-dose [180 mg/(kg·d)],and low-dose [45 mg/(kg·d)].After intmgastric administration for 30 d,except for the control group,ligation of the anterior interventricular branch of the left descending coronary artery was performed to establish a mouse model of myocardial ischemia reperfusion-induced arrhythmia.Lead Ⅱ electrocardiograms were recorded,and the time of arrhythmia onset,duration of arrhythmia,and incidences of ventricular premature (VP) beats,ventricular tachycardia (VT),and mortality in each group were examined and recorded at different reperfusion time points (15 min,30 min,1 h,2 h,4 h,and 24 h).Enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of atrial natriuretic peptide (ANP),brain natriuretic peptide (BNP),and cardiac troponin Ⅰ (cTn-Ⅰ) in plasma.Superoxide dismutase (SOD) and malondialdehyde (MDA) levels in myocardial tissue were determined using corresponding test kits.The results showed that Dendrobium candidum could shorten the recovery time after arrhythrnia and significantly decrease the arrhythmia score.The ANP,BNP,and cTn-Ⅰ levels in plasma of the rats with ischemia reperfusion-induced arrhythmia were increased,the SOD level in myocardium was decreased,and the MDA level was increased.Dendrobium candidum could inhibit these effects.This study indicated that Dendrobium candidum showed a protective effect on ischemia reperfusion-induced arrhythmia,and the mechanism might involve its antioxidant activity.关键词
铁皮石斛/缺血再灌注/心律失常/氧化应激Key words
Dendrobium candidum/ischemia reperfusion/arrhythmia/oxidative stress引用本文复制引用
翟旭峰,王瑾,郑爽,肖小春,娄勇军,马忠华,李年生..铁皮石斛对缺血再灌注诱导的心律失常的保护作用[J].现代食品科技,2017,33(7):1-8,8.基金项目
中南大学中央高校基本科研业务费专项资金资助(2016zzts497) （2016zzts497）
中南大学大学生创新课题资助项目(201610533575) （201610533575）
