现代食品科技2017，Vol.33Issue(7)：17-24,16,9.DOI:10.13982/j.mfst.1673-9078.2017.7.003
益生菌制剂对抗生素诱导腹泻模型小鼠肠道菌群的恢复
The Effects of Probiotics on the Restoration of Intestinal Microflora in a Mouse Model of Antibiotic-induced Diarrhea
摘要
Abstract
In order to evaluate the effects ofprobiotics on the restoration of intestinal microflora in a mouse model of diarrhea,22.4 g/(kg/d) ampicillin was administered by gavage to constmct the mouse model of diarrhea,and the blank control group was given saline at the same dosage.After the model was successfully constructed,low-,medium-,and high-dose probiotics were used to treat the mice to restore their intestinal microflora.The effect of probiotics on the loose stool rate,degree of loose stool,and diarrhea index were observed after 14 days of treatment.The aseptically collected fecal samples of mice at days 0,7,and 14 after treatment were analyzed,and changes in the target intestinal flora were determined using denaturing gradient gel electrophoresis (DGGE) and plate counting methods.After the completion of probiotic treatment,the fecal samples of mice were collected aseptically at days 0,7,and 14,and real-time fluorescent quantitative PCR (FQ-PCR) was carried out for the quantitative determination of exogenous probiotics.The results showed that probiotics not only mitigated diarrhea in mice and promoted mouse growth,but also exhibited a significant effect on the restoration of intestinal flora in mice with diarrhea.The results from FQ-PCR analysis indicated that the exogenous probiotics Lactobacillus acidophilus NCFM,Bifidobacterium lactis Bi-07,and Lactobacillus rhamnosus HN001 were able to colonize and smvive after the administration of probiotics was stopped,but showed a certain downward trend over time.关键词
益生菌/肠道菌群/BALB/c小鼠/腹泻模型Key words
probiotics/intestinal microflora/BALB/c mouse/diarrhea model引用本文复制引用
金鑫,葛亚中,敬璞,游丽君..益生菌制剂对抗生素诱导腹泻模型小鼠肠道菌群的恢复[J].现代食品科技,2017,33(7):17-24,16,9.基金项目
广东省自然科学基金项目(2014A030313242) （2014A030313242）
中央高校科研业务费项目(2015ZZ110) （2015ZZ110）
