现代食品科技2017，Vol.33Issue(7)：43-49,7.DOI:10.13982/j.mfst.1673-9078.2017.7.007
藻胆蛋白在大肠杆菌中的重组表达及其光谱学性质与抗氧化活性研究
Optimization of Expression of Recombinant Phycobiliproteins in Escherichia coil and Assessment of Their Spectral Properties and Antioxidant Activity
摘要
Abstract
Recombinant phycobiliproteins were expressed in Escherichia coll The overexpressed phycobiliproteins were isolated and purified,and their spectral properties and antioxidant activities were determined.The optimal conditions for the induction of recombinant phycobiliproteins with isopmpyl-beta-D-thiogalactopyranoside (IPTG) were identified as following:adding 0.1 mmol/L of IPTG and 5 mmol/L of 5-aminolevulinic acid (5-ALA) into the growth medium at an optical density at 600 nm (OD600) of 0.95 and then incubating the cells at 18 ℃ for 24 h.Under these conditions,the expression level of the phycobiliproteins was 149.5 mg/L.The optimal conditions for the induction of recombinant phycobiliproteins with lactose were adding 1.0 g/L of lactose and 5 mmol/L of 5-ALA to the bacterial culture at an OD600 of 1.2,followed by incubation at 18 ℃C for 26 h.The expression level in this case was 120.9 mg/L.The recombinant phycobiliproteins exhibited maximum absorbance at 550 nm and maximum emission at 562 nm.Compared to recombinant proteins obtained upon induction by IPTG,the recombinant proteins obtained upon induction by lactose had a higher absorbance at 550 nm,higher fluorescence emission at 562 nim,and higher hydroxyl radical and superoxide anion-scavenging activities.关键词
藻胆蛋白/乳糖/IPTG/诱导表达/抗氧化活性Key words
phycobiliprotein/lactose/isopropyl-beta-D-thiogalactopyranoside (IPTG)/inducible expression/antioxidant activity引用本文复制引用
朱晓文,陈华新,齐宏涛,姜鹏,李富超,李荣贵..藻胆蛋白在大肠杆菌中的重组表达及其光谱学性质与抗氧化活性研究[J].现代食品科技,2017,33(7):43-49,7.基金项目
国家自然科学基金资助项目(41276164) （41276164）
中国科学院战略性先导科技专项(XDA11030404) （XDA11030404）
863计划项目(2014AA093505、2014AA093501) （2014AA093505、2014AA093501）
