现代食品科技2017，Vol.33Issue(7)：105-111,62,8.DOI:10.13982/j.mfst.1673-9078.2017.7.016
原儿茶醛对阪崎克罗诺肠杆菌的抑制作用及机理
Antibacterial Activity of Protocatechualdehyde against Cronobacter sakazakii and its Possible Mechanism of Action
摘要
Abstract
To investigate the antibacterial activity of protocatechualdehyde (PC),the minimum inhibitory concentrations (MICs) of PC against C.sakazakii strains and the effect of PC on C.sakazakii growth curves were determined.The effect of PC on the cell morphology of C.sakazakii was observed under field emission scanning electron microscope.In addition,the impacts of PC on cell membrane integrity,intracellular pH (pHin),intracellular adenosine triphosphate (ATP) concentration and membrane potential were measured using multiple fluorescent probes to elucidate its possible antibacterial mechanism.The results showed that the MICs of PC against C.sakazakii strains ranged from 0.625 to 1.25 mg/mL,and the addition of PC significantly reduced the specific growth rate and the maximum optical density of C.sakazakii.Electronic microscopy observations indicated that C sakazakii cells exposed to PC had a wrinkled surface and lost the typical structure of Corynebacterium spp.In addition,PC treatments at concentrations of 2MIC and 4MIC reduced cell integrity by 49.0％ and 50.7％,respectively,and caused a decrease in pHin of C sakazakii from 6.87 to 5.67 and 4.47,respectively;the PC treatments reduced the intracellular ATP concentration from 1.824 μnol/L to less than 0.01 μmol/L.In addition,cells treated with PC showed depolarized cell membranes.In conclusion,PC had good antibiotic activity against C sakazakii,and its possible mechanism of action was to affect the permeability of the cell membrane.PC has the potential to be used as a natural antibacterial agent in infant formula or other foods.关键词
阪崎克罗诺肠杆菌/原儿茶醛/细胞膜/胞内ATPKey words
Cronobacter sakazakii/protocatechualdehyde/cell membrane/intracellular adenosine triphosphate (ATP)引用本文复制引用
石超,郭都,张文婷,刘志远,郭晓,郝旭昇,杨逸菲,孙怡,夏效东..原儿茶醛对阪崎克罗诺肠杆菌的抑制作用及机理[J].现代食品科技,2017,33(7):105-111,62,8.基金项目
“十二五”国家科技支撑计划项目(2015BAD16B08) （2015BAD16B08）
“浙江省植物有害生物防控重点实验室-省部共建国家重点实验室培育基地(2010DS700124-ZM1608)”项目 （2010DS700124-ZM1608）
教育部“新世纪优秀人才支持计划”(NCET-13-0488) （NCET-13-0488）
