现代食品科技2017，Vol.33Issue(7)：261-269,9.DOI:10.13982/j.mfst.1673-9078.2017.7.037
转基因甜菜品系H7-1的数字PCR定量检测方法
Method for Quantitative Determination of Genetically Modified Sugar Beet Line H7-1 by Using Digital Polymerase Chain Reaction
摘要
Abstract
In order to achieve labeling management and accurate quantification of genetically modified sugar beet H7-1,primer pairs and probes based on the 5'flanking sequence and glutamine synthetase (GS) of H7-1 were designed,and a duplex digital polymerase chain reaction (dPCR) detection method for H7-1 was established.The specificity,sensitivity,precision,and accuracy of the developed method were examined.The results showed that the developed dPCR method was specific for line H7-1 detection.The limits of quantitation (LOQs) of the specific sequence and endogenous GS gene of line H7-1 in a 20-μL reaction system were 3.1 copies/μL and 6.3 copies/μL respectively.The limits of detection (LODs) of the specific sequence and endogenous GS gene of line H7-1 in a 20 μL reaction system were 0.6 copies/μL and 1.3 copies/μL respectively.The precision and accuracy were all in the acceptable range.This quantitative method does not rely on the establishment of a standard curve,and can be easily applied for accurate quantification of sugar beet H7-1.关键词
转基因甜菜/H7-1品系/数字PCRKey words
genetically modified sugar beet/line H7-1/digital polymerase chain reaction (dPCR)引用本文复制引用
刘二龙,李婷,高东微,刘津..转基因甜菜品系H7-1的数字PCR定量检测方法[J].现代食品科技,2017,33(7):261-269,9.基金项目
广东省科技计划项目(2014A040401029) （2014A040401029）
出入境检验检疫行业标准计划项目(2015B218k) （2015B218k）
广东出入境检验检疫局科技计划项目(2015GDK13) （2015GDK13）
