现代食品科技2017，Vol.33Issue(7)：280-288,260,10.DOI:10.13982/j.mfst.1673-9078.2017.7.039
QuEChERS-超高效液相色谱串联质谱法测定发酵黑茶中的10种真菌毒素
Determination of Ten Mycotoxins in Fermented Dark Tea by QuEChERS-Ultra-high-performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
A method was established for the determination of ten mycotoxins (aflatoxin B1 (AFB1),aflatoxin B2 (AFB2),aflatoxin (G1 (AFG1),aflatoxin G2 (AFG2),deoxynivalenol (DON),3-acetyldeoxynivalenol (3-AcDON),zearalenone (ZEN),T-2 mycotoxin (T-2),HT-2 toxin (HT-2),and ochratoxin A (OTA)) in fermented dark tea (Pu'er tea,Hunan dark tea,Guangxi Liupu tea,and Hubei old green tea) using (QuEChERS) ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).The samples were extracted with acetouitrile-water (84:16,V/V),and the extracted solutions were purified using the QuEChERS approach.The mycotoxins were then separated on an Agilent Zorbax Rrhd SB-C18 column (2.1 mm×100 mm,1.8 μm) using (0.1％ formic acid-5 mM ammonium acetate-water)/methanol as the mobile phase and detected by MS/MS under multiple reaction monitoring (MRM) mode.The mass spectrometry measurement was conducted using positive (ESI+) and negative (ESI-) electrospray ionization with an external standard.The results showed that ten mycotoxins had a good linear relationship within their own linear response ranges,with correlation coefficients (r) all ＞0.9995.The limits of quantitation (LOQ) (S/N＞10) were 0.1～1.0 μg/kg,and the average recoveries at three spike levels often mycotoxins ranged fiom 61.9％ to 120.3％,with a relative standard deviation (RSD,n=3) of 3.2％～16.1％.This newly developed method was successfully applied for the assessment of 61 commercial black tea samples,and mycotoxins were detected in several batches of samples.Based on these results,the developed method is reliable,simple,and accurate,and is suitable for the rapid analysis of multiple mycotoxin contaminants in fermented dark tea.关键词
真菌毒素/超高效液相色谱-串联质谱/发酵黑茶/多残留检测Key words
mycotoxins/ultra-high-performance liquid chromatography-tandem mass spectrometry/fermented dark tea/multi-residue determination引用本文复制引用
刘妍,陈坚,谭贵良,刘子雄,李向丽..QuEChERS-超高效液相色谱串联质谱法测定发酵黑茶中的10种真菌毒素[J].现代食品科技,2017,33(7):280-288,260,10.基金项目
广东省高等学校优秀青年教师培养计划资助项目(Yq2013196) （Yq2013196）
国家质检总局科技计划项目(2014QK051) （2014QK051）
广东省食药局科技计划项目(2015ZX07) （2015ZX07）
中山市科技计划项目(2014A2FC247、2015B2297) （2014A2FC247、2015B2297）
