现代食品科技2017，Vol.33Issue(7)：315-319,279,6.DOI:10.13982/j.mfst.1673-9078.2017.7.044
功能性核酸增强Hemin催化活性用于乳制品中四环素检测研究
Tetracycline Detection in Dairy Products by Using Hemin with Functional Nucleic Acid-enhanced Catalytic Activity
摘要
Abstract
In order to establish a convenient and effective method for the colorimetric detection of tetracycline (TET) residues,guanine-rich single stranded deoxyribonucleic acid (G-ssDNA) was first used to enhance the horseradish peroxidase activity of hemin,which oxidized 3,3',5,5'-tetramethylbenzidine (TMB) to form a yellow product.With the addition of TET in the system,the catalytic activity of hemin was suppressed,and the substrate was oxidized to form a blue product.The TET content could be determined by measuring the change in the absorption spectra of the reaction products.Under optimal measurement conditions,the proposed method has a detection limit of 5.69 nM,and a linear range from 10 nM to 100 nM,with a correlation coefficient of 0.994.The specificity test confirmed that other related antibiotics did not show significant cross-reactivity with the TET measurement.This method was used to detect TET in milk samples,and the spiked recoveries for TET detection were in the range of 92％ to 99％.The advantages of the method established in this work include high specificity and convenient operation.In addition,the method allows for rapid TET detection without relying on large instruments,and thus can be widely used for the detection of TET residues in dairy products.关键词
四环素/氯化血红素/功能性核酸/检测Key words
tetracycline tablets/hemin/functional nucleic acids/detection引用本文复制引用
石彬,王啸,吴远根..功能性核酸增强Hemin催化活性用于乳制品中四环素检测研究[J].现代食品科技,2017,33(7):315-319,279,6.基金项目
国家自然科学基金项目(21565009) （21565009）
贵州省科学技术基金项目(黔科合基础[2016]1403号) （黔科合基础[2016]1403号）
贵州省科技厅科技合作计划(黔科合LH字[2015] 7682号) （黔科合LH字[2015] 7682号）
贵州省教育厅招标项目(黔教台KY宇[2014] 261号) （黔教台KY宇[2014] 261号）
