食品科学2017，Vol.38Issue(13)：26-33,8.DOI:10.7506/spkx1002-6630-201713005
薏米中多酚化合物的分离纯化及抗氧化活性分析
Isolation and Purification of Polyphenols from Adlay and Their Antioxidant Activity
摘要
Abstract
In this study,Sephadex LH-20 column chromatography was used to separate and purify six polyphenol fractions from adlay.The Folin-Ciocalteu method was used for the determination of the total phenolic content in each fraction.Three different antioxidant assays namely,oxygen radical absorbance capacity (ORAC),peroxyl radical scavenging capacity (PSC) and cellular antioxidant activity (CAA) were carried out to evaluate antioxidant capacity of six fractions.Among these,three fractions (Ⅱ,Ⅲ and V) with the strongest antioxidant activity were purified by semi-preparative HPLC to obtain four polyphenol compounds,whose antioxidant activity was investigated.The results indicated that polyphenols were mainly distributed in fraction Ⅱ,Ⅲ and V,with total phenolic contents of (30.56 ± 2.25),(17.40 ± 2.76) and (25.18 ± 1.10) mg GAE/100 g md.It was also found that bound phenolic compounds accounted for more than one third of the total phenolics in adlay.The purified phenolics were identified as N1,N5-double (p-coumaroyl) spermine,p-coumaric acid,ferulic acid and rutin,with N1,N5-double (p-coumaroyl) spermine being the major free phenolic compound and ferulic acid being the major bound one,which also existed in a small quantity in the bound form.All these phenolics were the main active compounds responsible for the strong antioxidant activity of polyphenols from adlay.关键词
薏米/多酚/分离纯化/抗氧化活性Key words
adlay/polyphenols/isolation/antioxidant activity分类
轻工纺织引用本文复制引用
张怡一,徐茜,陈琳,姚轶俊,鞠兴荣,王立峰..薏米中多酚化合物的分离纯化及抗氧化活性分析[J].食品科学,2017,38(13):26-33,8.基金项目
国家自然科学基金面上项目(31571766) （31571766）
江苏省自然科学基金面上项目(BK20141485) （BK20141485）
江苏省高校自然科学重大项目(15KJA550002) （15KJA550002）
