食品科学2017，Vol.38Issue(13)：60-65,6.DOI:10.7506/spkx1002-6630-201713010
脂质体在婴儿体外胃肠道消化的膜结构稳定性
Membrane Stability of Liposomes during in Vitro Simulated Infant Gastrointestinal Digestion
摘要
Abstract
In order to study the effect of liposomes on gastric oxidative stress and the lipolysis kinetics of liposomes during gastric and small intestinal digestion,an in vitro infant gastrointestinal system was established,with its adult counterpart as control.Results showed that the average diameter of liposomes was significantly decreased and the negative zeta potential was increased after digestion.The oxidation degree of liposomal membrane,which was characterized by thiobarbituric acid reactive substances (TBARS),significantly increased after gastric digestion.Free fatty acids were released continuously during stimulated intestinal digestion,and the rate of lipolysis in the infant gastrointestinal system (38％) was obviously higher than that in the adult one (80％).The release profiles fitted first-order kinetics.Moreover,Fourier transform infrared spectroscopy (FT-IR) showed that the peaks of characteristic groups in phospholipids (C=O,P=O and-CH2) changed after digestion,and the liposomal membrane in the infant gastrointestinal system was damaged less seriously than in the adult one.This study can provide a theoretical guidance for liposomes as a nutrient carrier in infant formula.关键词
脂质体/体外消化/婴儿/膜结构/稳定性Key words
liposome/in vitro digestion/infant/membrane structure/stability分类
轻工纺织引用本文复制引用
冯炎雯,李娜,徐纪璇,邬琰泽,韩剑众,刘玮琳..脂质体在婴儿体外胃肠道消化的膜结构稳定性[J].食品科学,2017,38(13):60-65,6.基金项目
国家自然科学基金青年科学基金项目(31401482) （31401482）
浙江省食品科学与工程重中之重一级学科开放基金项目(JYTSP20142011) （JYTSP20142011）
浙江省公益技术应用研究计划项目(2016C32060) （2016C32060）
浙江省现代食品安全与营养协同创新中心项目(2017SICR103) （2017SICR103）
