食品科学2017，Vol.38Issue(13)：167-173,7.DOI:10.7506/spkx1002-6630-201713028
黑灵芝多糖体内抗炎活性及对甘露糖受体表达的影响
In Vivo Anti-Inflammatory Activity of a Polysaccharide from Ganoderma atrum and Its Effect on Mannose Receptor Expression
摘要
Abstract
Objective:To explore the in vivo anti-inflammatory activity of a water-soluble polysaccharide purified from Ganoderma atrum,named PSG-1,and its effect on mannose receptor (MR) expression.Methods:In the present study,lipopolysaccharide (LPS) via intraperitoneal injection was applied to establish a mouse model of inflammation.Mice were randomly divided into five groups:control group,LPS group,high-dose PSG-1 (100 mg/(kg·d)) + LPS group,mediumdose PSG-1 (50 mg/(kg·d)) + LPS group and low-dose PSG-1 (25 mg/(kg·d)) + LPS group.MR expression,phagocytosis and the level of reactive oxygen species (ROS) in maerophages were analyzed by flow cytometry.The levels of tumor necrosis factor-α (TNF-α),interleukin-1β (IL-1β) and interleukin-6 (IL-6) in serum were determined by enzyme-linked immunosorbent assay (ELISA).Results:Compared with the control group,MR expression on the macrophage surface was decreased in the LPS group.In contrast,MR expression on the macrophage surface was significantly increased in the PSG-1 + LPS group in comparison with the LPS group (P ＜ 0.01).Macrophage phagocytosis and the level of ROS in the LPS group were higher than in the control group,which were significantly decreased after administration of PSG-1 (P＜0.05,P＜0.01).After mice were administrated with LPS,the levels of TNF-α,IL-1β and IL-6 were significantly increased in serum in comparison with the control group (P ＜ 0.01).Compared with the LPS group,the expression level of TNF-α was significantly decreased after administration of high-dose PSG-1 (P ＜ 0.05),but the expression levels of IL-1β and IL-6 did not significantly change.Conclusion:PSG-1 had an anti-inflammatory effect,being able to partly suppress inflammation induced by LPS in vivo,by promoting MR expression on the peritoneal macrophage surface in mice and suppressing macrophage polarization to M1 phenotype.关键词
黑灵芝多糖/脂多糖/巨噬细胞/甘露糖受体/吞噬功能/炎症因子/极化Key words
Ganoderma atrum polysaccharide/lipopolysaccharide/macrophage/mannose receptor/phagocytosis/cytokine/polarization分类
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张妍淞,李文娟,汤小芳,聂少平,谢明勇..黑灵芝多糖体内抗炎活性及对甘露糖受体表达的影响[J].食品科学,2017,38(13):167-173,7.基金项目
国家自然科学基金地区科学基金项目(31560460) （31560460）
江西省科技厅自然科学基金项目(20151512041185) （20151512041185）
食品科学与技术国家重点实验室项目(SKLF-QN-201509) （SKLF-QN-201509）
