食品科学2017，Vol.38Issue(13)：238-243,6.DOI:10.7506/spkx1002-6630-201713039
副干酪乳杆菌L9对小鼠肠道短链脂肪酸含量的影响
Effect of Lactobacillus paracasei L9 on the Content of Intestinal Short Chain Fatty Acids in Healthy Mice
王晓成 1张明 2陈善斌 3孙亚楠 3任发政 3仝其根1
作者信息
- 1. 北京农学院食品科学与工程学院,北京 102206
- 2. 北京工商大学食品学院,北京 100048
- 3. 教育部北京市共建功能乳品重点实验室,北京 100083
- 折叠
摘要
Abstract
Objective:To evaluate the effect of Lactobacillus paracasei L9 on the content of intestinal short chain fatty acids (SCFAs) in healthy mice.Methods:Fifty three-week-old BALB/c male mice were randomly divided into 5 groups:high dose,medium dose,low dose,positive control and negative control groups.After continuous administration at a dose of 0.2 mL/day per mouse for 21 days,mouse feces were collected,and the cecal contents were also examined after sacrificing the mice on day 21.The changes in SCFAs concentrations of samples were analyzed by gas chromatography,and RT-PCR was used to analyze the changes in the intestinal flora.Results:The concentration of short-chain fatty acids including acetic acid,propionic acid and butyric acid increased significantly (P ＜ 0.05),when compared with those observed before administration.The counts of Bifidobaceterium spp.and Lactobacillus and the expression level of butyryl-CoA:acetate CoA-transferase gene (CoAT) were increased significantly (P ＜ 0.05) in feces of mice on day 21.For the cecal contents,Bifidobaceterium spp.count and the expression level of CoATin low-dose and high-dose groups showed a significant difference (P ＜ 0.05) those in normal saline group.Conclusion:Lactobacillus paracasei L9 can adjust the intestinal flora and promote the production of short chain fatty acids in the intestine of healthy mice.Compared with commercial strain Lactobacillus casei strain Shirota,L9 has a better effect on promoting the yield of propionic acid and butyric acid and the growth of Bifidobaceterium spp.in the intestine.关键词
副干酪乳杆菌L9/短链脂肪酸/肠道菌群Key words
Lactobacillus paracasei L9/short chain fatty acids (SCFAs)/intestinal microflora分类
轻工纺织引用本文复制引用
王晓成,张明,陈善斌,孙亚楠,任发政,仝其根..副干酪乳杆菌L9对小鼠肠道短链脂肪酸含量的影响[J].食品科学,2017,38(13):238-243,6.
