食品科学2017，Vol.38Issue(13)：299-305,7.DOI:10.7506/spkx1002-6630-201713048
免疫学方法在食源性副溶血性弧菌检测中的应用研究进展
Recent Advances in Application of Immunological Methods in Detection of Foodborne Vibiro parahaemolyticus
摘要
Abstract
The bacterial pathogen Vibrio parahaemolyticus (VP) can pollute a variety of foods,especially seafoods.Foodbome VP has surged as the primary pathogen in food safety incidents in China.Compared to traditional cultural methods and modern molecular techhniques,specific antigen-antibody recognition-based immunological identification and subtyping strategies exhibit superiority in efficiency,specificity,on-field applicability and simplicity of pre-treatment.In this paper,the category and preparation methods of anti-VP antibodies at present are outlined.Furthermore,we review the current applications of immunological methods such as immunomagnetic separation (IMS),enzyme-linked immunosorbent assay (ELISA),immunochromatographic assay (ICA),immunosensor and immunomicroscopy in the detection of foodborne VP,which will provide useful references for rapid detection of VP.关键词
食源性副溶血性弧菌/免疫磁珠分离/酶联免疫吸附测定/免疫层析测试/免疫传感器/免疫荧光显微术Key words
foodborne Vibrio parahaemolyticus/immunomagnetic separation (IMS)/enzyme-linked immunosorbent assay (ELISA)/immunochromatographic assay (ICA)/immunosensor/immunofluorescence microscopy (IFM)分类
轻工纺织引用本文复制引用
谢雪钦,刘舟..免疫学方法在食源性副溶血性弧菌检测中的应用研究进展[J].食品科学,2017,38(13):299-305,7.基金项目
厦门市科技计划项目(3502Z20154086) （3502Z20154086）
福建省自然科学基金青年科技人才创新项目(2014J01118) （2014J01118）
