食品科学2017，Vol.38Issue(14)：9-16,8.DOI:10.7506/spkx1002-6630-201714002
微紫青霉酸性木聚糖酶xynA基因的克隆与序列分析
Cloning and Bioinformatics Analysis of Acidophilic xynA Gene from Penicillium janthinellum
摘要
Abstract
Acidic xylanases have extensive application in feed and wine industries.The whole sequence of the gene xynA encoding acidic xylanase was amplified from Penicilliumjanthinellum MA21601 by genome walking.A cDNA sequence was obtained through the elimination of introns by overlapping PCR and analyzed by bioinformatics.The whole sequence was about 720 bp in length with only one intron of 63 bp.The cDNA sequence was 657 bp long and putatively encoded a protein which contained a 28-amino acid (aa) signal peptide and a 190-aa mature peptide.The molecular weight of the protein was estimated to be about 20.61 kD,which had an isoelectric poim of 7.0.Bioinformatics analysis showed that XynA was a hydrophilic protein without disulfide bond.The amino acid sequence comparison of XynA with other fungal GH11 acidophilic xylanases indicated that the XynA had an identified specific recognition site of Asp,which displayed a β-jelly-roll architecture as a conserved region which was the characteristic of the GH11 family xylanases.The recombinant xylanase was successfully expressed in Escherichia coli with a specific activity of up to 220.5 U/mg.关键词
微紫青霉/酸性木聚糖酶/基因克隆/生物信息学分析Key words
Penicillium janthinellum/acidophilic xylanase/gene cloning/bioinformatics analysis分类
生物科学引用本文复制引用
侯洁,李琴,熊科,徐友强,李秀婷..微紫青霉酸性木聚糖酶xynA基因的克隆与序列分析[J].食品科学,2017,38(14):9-16,8.基金项目
国家自然科学基金面上项目(31371723) （31371723）
