食品科学2017，Vol.38Issue(14)：24-29,6.DOI:10.7506/spkx1002-6630-201714004
苯基乳酸耐受性大肠杆菌的筛选及其高产苯基乳酸特性
Screening of Phenyllactic Acid-Resistant Escherichia coli and Its Application in the Synthesis of Phenyllactic Acid
摘要
Abstract
Screening phenyllactic acid (PLA)-resistant strains enables effective reduction of the inhibitory effect of PLA on the synthesis process and thus an increase in PLA production.In this report,Escherichia coli BL21 (DE3) was mutagenized by UV irradiation and screened for a mutant resistant to PLA,E.coli Z2016 (CCTCC Accession No.M2016332).E.coli Z2016was used the host strain to construct recombinant strains E.coli Z2016 pET-28a-ldhY52V and E.coli Z2016 pET-28a-ldhL for the synthesis of D-and L-PLA.The results showed that E.coli Z2016 was able to grow normally in a medium containing 1 g/L D-PLA.The production of D-and L-PLA by the recombinant strains were 6.75 and 6.97 g/(L·h),which were increased by 14.02％ and 8.95％ as compared to those produced by the original strain,respectively.During 120 min of fed-batch fermentation,the production of D-PLA by E.coli Z2016 pET-28a-ldhY2V was 20.02 g/L with a conversion rate of 90.97％,which was increased by 22.17％ as compared to that produced by the control.The L-PLA concentration produced by E.coli Z2016 pET-28a-ldhL was 20.87 g/L,which was increased by 16.85％ as compared to produced by the control,with a conversion rate of 91.24％.Accordingly,the use of PLA-resistant strains provided an effective method to increase PLA production.关键词
紫外诱变/苯基乳酸/苯基乳酸耐受菌株/大肠杆菌/全细胞转化Key words
UV mutagenesis/phenyllactic acid/phenyllactic acid-resistant strain/E.coli/whole-cell transformation分类
轻工纺织引用本文复制引用
徐艳,郭倩,朱益波,王立梅,齐斌..苯基乳酸耐受性大肠杆菌的筛选及其高产苯基乳酸特性[J].食品科学,2017,38(14):24-29,6.基金项目
国家自然科学基金面上项目(31470092) （31470092）
国家自然科学青年科学基金项目(31501459) （31501459）
江苏省“六大高峰人才”资助计划项目(NY-021) （NY-021）
