食品科学2017，Vol.38Issue(14)：64-70,7.DOI:10.7506/spkx1002-6630-201714010
脉冲式添加氮源对耐温微藻Desmodesmus sp.F51细胞生长和细胞组成的影响
Effect of Nitrate Pulse-Feeding Cultivation on Cell Growth and Cell Composition of Thermo-Tolerant Desmodesmus sp.F51
摘要
Abstract
The aim of the present study was to investigate the effect of nitrate pulse feeding on the cell growth and cell composition of Desmodesmus sp.F51.Under nitrate pulse-feeding cultivation,the protein content of cells of Desmodesmus sp.FS1 decreased from (560 ± 16) mg/g to (456 ± 17) mg/g,while the contents of carbohydrate and lipid increased from (209 ± 13) mg/g to (310 ± 12) mg/g and (98 ± 3) mg/g to (120 ± 4) mg/g,respectively.By investigating the changes in carotenoid,lipid and carbohydrate compositions,it appeared that nitrate pulse feeding could promote β-carotene biosynthesis and enhance the bioconversion of α-carotene to lutein.Lutein accumulation was positively associated with polyunsaturated fatty acid formation.Desmodesmus sp.F51 tended to accumulate carbohydrate as an energy storage product after 3 days of cultivation under nitrate pulse-feeding conditions,and the major accumulated monosaccharide was glucose (75.7 ±1.4)％.Therefore,the nitrate-pulse feeding strategy is a highly promising approach to simultaneously produce lutein and carbohydrate in Desmodesmus sp.F51.关键词
微藻/流加培养/细胞组成/叶黄素/碳水化合物Key words
microalgae/fed-batch culture/cell composition/lutein/carbohydrate分类
生物科学引用本文复制引用
谢友坪,赵旭蕊,阳需求,卢英华,郑向南,陈剑锋..脉冲式添加氮源对耐温微藻Desmodesmus sp.F51细胞生长和细胞组成的影响[J].食品科学,2017,38(14):64-70,7.基金项目
国家自然科学基金应急管理项目(41641050) （41641050）
福建省青年科技人才创新基金资助项目(2016J05077) （2016J05077）
福建省海洋高新产业发展专项(闽海洋高新[2016]15号) （闽海洋高新[2016]15号）
福州市市校(院所)科技合作项目(2015-G-69) （院所）
