食品科学2017，Vol.38Issue(14)：290-296,7.DOI:10.7506/spkx1002-6630-201714045
外源β-葡萄糖苷酶处理结合异烟酸-吡唑啉酮分光光度法测定橡胶籽中氰化物含量
Spectrophotometric Determination of Cyanide Content in Rubber Seeds Using Isonicotinic Acid-Pyrazolone after Exogenous β-Glucosidase Pretreatment
摘要
Abstract
This study investigated the determination of the residual cyanide content of rubber seeds detoxified by different methods by a spectrophotometric method using isonicotinic acid-pyrazolone with and without exogenous β-glucosidase pretreatment.The results showed that cyanide was undeteetable or detectable at a level lower than the actual one by the spectrophotometric method without exogenous β-glucosidase pretreatment,while it was detected at a significantly higher level with the sample pretreatment.The optimized pretreatment conditions were 3.35 h soaking at 45.06 ℃ and pH 5.56 with 24.11 U/100 mL of β-glucosidase added,under which,the predicted cyanide content of rubber seeds was 28.79 μg/g.The recovery of spiked samples ranged from 85.02％ to 92.04％.The proposed method was accurate and reliable.关键词
异烟酸-吡唑啉酮分光光度法/β-葡萄糖苷酶/橡胶籽/氰苷/热处理Key words
s: isonicotinic acid-pyrazolone spectrophotometry/β-glucosidase/rubber seed/cyanide/heat treatment分类
化学化工引用本文复制引用
张烨,王珂,刘石生..外源β-葡萄糖苷酶处理结合异烟酸-吡唑啉酮分光光度法测定橡胶籽中氰化物含量[J].食品科学,2017,38(14):290-296,7.基金项目
国家自然科学基金地区科学基金项目(31460407) （31460407）
