食品与发酵工业2017，Vol.43Issue(7)：13-19,7.DOI:10.13995/j.cnki.11-1802/ts.013871
耐高温葡萄糖异构酶重组菌发酵与转化条件研究
Research on fermentation and conversion conditions of recombinant strain harboring thermophilic glucose isomerase
摘要
Abstract
Thermophilic glucose isomerase (GI) can catalyze conversion of D-glucose to D-fructose at elevated temperature.The obtained high fructose corn syrup served as sweetener,is widely used in various food fields.Herein the fermentation and conversion conditions of E.coli BL21 (DE3)/pET28b/ToGI were studied.The results showed that the optimum fermentation should be performed at 28 ℃ by adding 0.1 mmol/L Isopropyl β-D-1-thiogalactopyranoside (IPTG) and 5 mmol/L Mn2+ Then,the amplified biotransformation reaction was conducted at the condition of 20 g/L wet cell,10 mmol/L Mn2+,200 g/L D-glucose and pH 7.5.After bioconversion at 95 ℃ for 4 h,the yield of D-fructose reached 57.34％.One-step production process of F55 high fructose corn syrup (HFCS) using this craft has the advantage of simplifying the subsequent step of enriching D-fructose,and it provided an important reference for industrial application of HFCS production using food safety expression system.关键词
Thermus oshimai葡萄糖异构酶/工程菌E.coli BL21(DE3)/pET28b/ToGI/D-葡萄糖/D-果糖/细胞催化转化Key words
Thermus oshimai glucose isomerase/engineering bacteria E.coli BL21 (DE3)/pET28b/ToGI/D-glucose/D-fructose/biotransformation引用本文复制引用
贾东旭,周霖,王腾,於淳安,廖承军,陈德水,王红艳,廖小颖,金利群..耐高温葡萄糖异构酶重组菌发酵与转化条件研究[J].食品与发酵工业,2017,43(7):13-19,7.基金项目
国家自然科学青年基金(31401527) （31401527）
中国博士后科学基金面上资助项目(2015M580522) （2015M580522）
浙江省博士后择优项目(BSH1502149) （BSH1502149）
浙江省教育厅项目(Y201431321) （Y201431321）
浙江省生物有机合成技术研究重点实验室开放课题(20170107) （20170107）
