食品与发酵工业2017，Vol.43Issue(7)：49-54,6.DOI:10.13995/j.cnki.11-1802/ts.013962
内生真菌β-D-葡萄糖醛酸苷酶固定化及其性质研究
Immobilization and properties of a high-selectivity β-D-glucuronidase from endophytic fungus Chaetomium globosum S108
摘要
Abstract
A high-selectivity β-D-glucuronidase from Endophytic Fungus Chaetomium globosum S108 was immobilized into matrix with sodium alginate as embedding material and glutaraldehyde as crosslinking agent.The optimal immobilized conditions were achieved with 1.5％ sodium alginate,1.5％ glutaraldehyde,2％ CaCl2 and 3 500 U of enzyme load of per milliliter matrix for 30 min.In addition,some immobilized enzymology properties were studied.Compared with free enzyme,the optimum temperature and pH of immobilized enzyme were higher than those of free form,and temperature stability and pH stability of immobilized enzyme were enhanced obviously.After immobilization of β-D-glucuronidase,the Michaelis constant (Km) was increased,and the maximum reaction velocity (Vmax) was decreased respectively.Immobilized β-D-glucuronicdase enzyme activity still retained 71％ of its initial activity after consecutive 15 cycles.关键词
海藻酸钠/β-D-葡萄糖醛酸苷酶/甘草酸/固定化/内生真菌Key words
sodium alginate/β-D-glucuronicdase/glycyrrhizin/immobilization/Endophytic Fungu引用本文复制引用
闫宜江,肖依文,汪涯,吴文婷,常军,何巧璇,朱笃..内生真菌β-D-葡萄糖醛酸苷酶固定化及其性质研究[J].食品与发酵工业,2017,43(7):49-54,6.基金项目
国家自然科学基金项目(31260137) （31260137）
“赣鄱英才555工程”领军人才计划项目 （）
江西科技师范大学自然科学科研计划项目(2016XJZD001)资助 （2016XJZD001）
